Exogenous Hydrogen Sulfide Postconditioning Protects Isolated Rat Hearts From Ischemia/Reperfusion Injury Through Sirt1/PGC-1α Signaling Pathway

被引：0
作者：
Hu, Ming-zhu [1 ,2 ]
Zhou, Bo [2 ]
Mao, Hong-ya [3 ]
Sheng, Qiong [1 ]
Du, Bin [1 ]
Chen, Jun-liang [1 ]
Pang, Qing-feng [1 ]
Ji, Yong [2 ]
机构：
[1] Jiangnan Univ, Wuxi Med Sch, 1800 Lihu Rd, Wuxi 214122, Peoples R China
[2] Jiangnan Univ, Dept Anesthesiol, Affiliated Hosp, Wuxi 214000, Jiangsu, Peoples R China
[3] Wuxi 3 Peoples Hosp, Dept Anesthesiol, Wuxi, Peoples R China
来源：
INTERNATIONAL HEART JOURNAL | 2016年 / 57卷 / 04期
关键词：
Hydrogen sulfide; Gasotransmitter; EX-527; Cardioprotection; ISCHEMIA-REPERFUSION INJURY; REGULATOR; PROTECTS; OXIDATIVE STRESS; MITOCHONDRIAL BIOGENESIS; NUCLEAR TRANSLOCATION; MYOCARDIAL-INFARCTION; SUPEROXIDE-DISMUTASE; INDUCED APOPTOSIS; SIRT1; ACTIVATION;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Sirt1 is a highly conserved nicotinamide adenine dinucleotide (NAD(+)) dependent histone deacetylase which plays an important role in heart diseases. Studies performed with Sirt1 activators indicated that it protects cells from ischemia/reperfusion (I/R) injury. The protective effects of H2S against I/R injury also have been recognized. Hence, the present study was designed to explore whether Sirt1/PGC-1 alpha a participates in the protection of exogenous H2S postconditioning against FR injury in isolated rat hearts. Isolated rat hearts were subjected to 30 minutes of global ischemia followed by 60 minutes of reperfusion after 20 minutes of equilibrium. During this procedure, the hearts were exposed to NaHS (10 mu mol/L) treatment in the absence or presence of the selective Sirt1 inhibitor EX-527 (10 mu mol/mon). NaHS exerted a protective effect on isolated rat hearts subjected to I/R, as shown by the improved expression of Sirt1/PGC-1 alpha associated with restoration of Sirt1 nuclear localization, cardiac function, decreased myocardial infarct size, decreased myocardial enzyme release, and several biochemical parameters, including up-regulation of the ATP and SOD levels, and doWn-regulation of the MDA level. However, treatment with EX-527 could partially prevent the above effects of NaHS postconditioning. These results indicate that H2S confers protective effects against I/R injury through the activation of Sirt1/PGC-1 alpha.
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页码：477 / 482
页数：6
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