MOLECULAR CHARACTERIZATION OF Cronobacter sakazakii ISOLATED FROM DIFFERENT HERBAL TEAS AND MIXTURES IN SERBIA

被引:0
作者
Josic, Dragana [1 ]
Stojanovic, Marija [2 ]
Lepsanovic, Zorica [3 ]
Katic, Vera [4 ]
机构
[1] Inst Soil Sci, Genet Lab, Teodora Drajzera 7, Belgrade, Serbia
[2] Ctr Food Anal, Belgrade, Serbia
[3] Mil Med Acad, Inst Epidemiol, Belgrade, Serbia
[4] Fac Vet Med, Belgrade, Serbia
来源
GENETIKA-BELGRADE | 2017年 / 49卷 / 03期
关键词
Cronobacter sakazakii; herbal tea; infant formula; RAPD; rep-PCR; 16S rRNA; SPP; ENTEROBACTER-SAKAZAKII; INFANT FORMULA; ANTIBIOTIC SUSCEPTIBILITY; GENETIC DIVERSITY; ERIC-PCR; PREVALENCE; FOOD; STRAINS; MILK; IDENTIFICATION;
D O I
10.2298/GENSR1703921J
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Cronobacter sakazakii is an important cause of human infections that can be serious and even fatal among premature neonates and immunocompromised adults or infants. Because of its high tolerance to osmotic stress, C. sakazakii is frequently isolated from dried foods, such as powdered infant formula and herbal teas. The aim of investigation was detection, identification and molecular characterization of Cronobacter sakazakii isolates from infant formula and various herbal teas collected from Serbian market and tested for import control. C. sakazakii was not detected in any of the 360 analysed samples of powdered infant formula. However, 192 out of 520 samples of herbal teas tested were positive for C. sakazakii (37.1%). The high prevalence was observed in teas for children (51.6%) and in "baby" teas (44.1%), followed by medicinal teas (38%). The largest one-herb-teas group (221 samples) contained 72 C. sakazakii-positive samples (32.6%) and involved Sennae folium, Althaeae radix, Menthae piperitae folium, Chamomilae flos and Urticae folium teas. Molecular characterization of isolated C. sakazakii from different herbal teas by rep-PCR, RAPD and 16S rRNA sequences analysis showed the high similarity to C. sakazakii NCTC 8155. Knowing this strain as one of the most pathogenic clinical strains, our results raise concern about the safety risks these foods pose to immunocompromised and healthy consumers, especially for babies and children.
引用
收藏
页码:921 / 934
页数:14
相关论文
共 47 条
[1]   Dry stress and survival time of Enterobacter sakazakii and other Enterobacteriaceae in dehydrated powdered infant formula [J].
Barron, Juncal Caubilla ;
Forsythe, Stephen J. .
JOURNAL OF FOOD PROTECTION, 2007, 70 (09) :2111-2117
[2]   Detection and frequency of Cronobacter spp. (Enterobacter sakazakii) in different categories of ready-to-eat foods other than infant formula [J].
Baumgartner, Andreas ;
Grand, Marius ;
Liniger, Marianne ;
Iversen, Carol .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2009, 136 (02) :189-192
[3]   Urinary tract infection due to Enterobacter sakazakii [J].
Bhat, Gopalkrishna K. ;
Anandhi, R. S. ;
Dhanya, V. C. ;
Shenoy, Shalini M. .
INDIAN JOURNAL OF PATHOLOGY AND MICROBIOLOGY, 2009, 52 (03) :430-431
[4]   Isolation of Cronobacter spp. (Enterobacter sakazakii) from artisanal mozzarella [J].
Casalinuovo, Francesco ;
Rippa, Paola ;
Battaglia, Luciana ;
Parisi, Nicola .
ITALIAN JOURNAL OF FOOD SAFETY, 2014, 3 (01) :29-32
[5]   Genotypic and phenotypic analysis of Enterobacter sakazakii strains from an outbreak resulting in fatalities in a neonatal intensive care unit in France [J].
Caubilla-Barron, J. ;
Hurrell, E. ;
Townsend, S. ;
Cheetham, P. ;
Loc-Carrillo, C. ;
Fayet, O. ;
Prere, M. -F. ;
Forsythe, S. J. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (12) :3979-3985
[6]   MOLECULAR TYPING OF CRONOBACTER STRAINS FROM FOOD IN CHINA BY ENTEROBACTERIAL REPETITIVE INTERGENIC CONSENSUS SEQUENCE PCR (ERIC-PCR) AND SEQUENCE ANALYSIS OF THE gyrB GENE [J].
Chen, Wanyi ;
Ai, Lianzhong ;
Yang, Jielin ;
Ren, Jing ;
Li, Yunfei ;
Guo, Benheng .
JOURNAL OF FOOD SAFETY, 2013, 33 (04) :405-412
[7]   Postsurgical osteomyelitis caused by Enterobacter sakazakii in a healthy young man [J].
Corti, G. ;
Panunzi, I. ;
Losco, M. ;
Buzzi, R. .
JOURNAL OF CHEMOTHERAPY, 2007, 19 (01) :94-96
[8]   Characterization of a collection of Enterobacter sakazakii isolates from environmental and food sources [J].
Drudy, Denise ;
O'Rourke, Michele ;
Murphy, Mary ;
Mullane, Niall R. ;
O'Mahony, Rebecca ;
Kelly, Lorraine ;
Fischer, Matthias ;
Sanjaq, Suhad ;
Shannon, Pauline ;
Wall, Patrick ;
O'Mahony, Micheal ;
Whyte, Paul ;
Fanning, Seamus .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2006, 110 (02) :127-134
[9]  
FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x
[10]   Enterobacter sakazakii in food and beverages (other than infant formula and milk powder) [J].
Friedemann, Miriam .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2007, 116 (01) :1-10