Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli

被引：12

作者：

Ge, BS

Tang, ZH

Lin, LP

Ren, YH

Yang, Y

Qin, S

机构：

[1] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[3] Chinese Acad Sci, Inst Mat Med, Shanghai 201203, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 11期

关键词：

allophycocyanin; Escherichia coli; protein purification; recombinant allophycocyanin; tumor inhibition;

D O I：

10.1007/s10529-005-5794-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amylose affinity column. The purity of product was greater than 98% and yielded an average of 5.5 g kg(-1) dry cells. Recombinant allophycocyanin significantly inhibited H-22 hepatoma (p (0.01) in mice with inhibition rates ranging from 36% to 62% with doses from 6.25 to 50 mg kg(-1) d(-1).

引用

页码：783 / 787

页数：5

共 7 条

[1]

Estrada JEP, 2001, FARMACO, V56, P497

[2]

QIN S, 2005, IN PRESS J APPL PHYC

[3] C-Phycocyanin, a selective cyclooxygenase-2 inhibitor, induces apoptosis in lipopolysaccharide-stimulated RAW 264.7 macrophages [J].