Electrodeformation for single cell mechanical characterization

被引：71

作者：

Chen, Jian ^{[1
]}

Abdelgawad, Mohamed ^{[2
]}

Yu, Liming ^{[2
]}

Shakiba, Nika ^{[1
]}

Chien, Wei-Yin ^{[1
]}

Lu, Zhe ^{[2
]}

Geddie, William R. ^{[3
]}

Jewett, Michael A. S. ^{[4
]}

Sun, Yu ^{[1
,2
]}

机构：

[1] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5S 3G8, Canada

[2] Univ Toronto, Dept Mech & Ind Engn, Toronto, ON M5S 3G8, Canada

[3] Univ Toronto, Dept Pathol, Toronto, ON M5G 2C4, Canada

[4] Princess Margaret Hosp, Dept Surg Oncol, Toronto, ON M5G 2M9, Canada

来源：

JOURNAL OF MICROMECHANICS AND MICROENGINEERING | 2011年 / 21卷 / 05期

基金：

加拿大自然科学与工程研究理事会;

关键词：

RED-BLOOD-CELLS; VISCOELASTIC PROPERTIES; BIOPHYSICAL PROPERTIES; ERYTHROCYTE-MEMBRANES; DIELECTRIC-PROPERTIES; CANCER; BIOMECHANICS; DEFORMATION; ELASTICITY; SEPARATION;

D O I：

10.1088/0960-1317/21/5/054012

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

This paper presents the use of electrodeformation as a method for single cell mechanical characterization in which mechanical properties of SiHa and ME180 cells (two cervical cancer cell lines) were quantified. Cells were directly placed between two microelectrodes with a rectangular ac electric field applied, and cell deformation was recorded under certain experimental conditions. Numerical simulations were performed to model cell electrodeformation based on the Maxwell stress tensor formulation. In these simulations, effects of cell electrical property variations on their electrodeformed behavior were investigated. By comparing the measured morphological changes with those obtained from numerical simulations, we were able to quantify Young's modulus of SiHa cells (601 +/- 183 Pa) and ME180 cells (1463 +/- 649 Pa). These values were consistent with Young's modulus values (SiHa: 400 +/- 290 Pa and ME180: 1070 +/- 580 Pa) obtained from conventional micropipette aspiration.

引用

页数：11

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