Characterization and stability studies on surfactant, detergent and oxidant stable α-amylase from marine haloalkaliphilic Saccharopolyspora sp. A9

被引：83

作者：

Chakraborty, Samrat ^{[2
]}

Khopade, Abhijeet ^{[2
]}

Biao, Ren ^{[3
]}

Jian, Wang ^{[3
]}

Liu, Xiang-Yang ^{[3
]}

Mahadik, Kakasaheb ^{[2
]}

Chopade, Balasaheb ^{[4
]}

Zhang, Lixin ^{[3
]}

Kokare, Chandrakant ^{[1
,2
]}

机构：

[1] STES Sinhgad Inst Pharm, Pune 411041, Maharashtra, India

[2] Bharati Vidyapeeth Univ, Poona Coll Pharm, Dept Pharmaceut Biotechnol, Pune 411038, Maharashtra, India

[3] Chinese Acad Sci, Key Lab Pathogen Microbiol & Immunol, Inst Microbiol, Beijing 100101, Peoples R China

[4] Univ Pune, Inst Bioinformat & Biotechnol, Pune 411007, Maharashtra, India

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2011年 / 68卷 / 01期

关键词：

alpha-Amylase; Halophilic; Thermostable; Surfactant; Oxidant stable; BACILLUS SP; RAW STARCH; ENZYMATIC-PROPERTIES; ALKALINE PROTEASE; PURIFICATION; MICROORGANISMS; ISOLATE;

D O I：

10.1016/j.molcatb.2010.09.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A halopalkaliphilic marine Saccharopolyspora sp stain A9 with an ability to produce surfactants oxidant and detergent stable a-amylase was Isolated from marine sediments collected from west coast of India The a-amylase from strain A9 was purified to homogeneity with the aid of ammonium sulfate precipitation and gel filtration chromatography by using Sephadex G-75 insoluble corn starch and sephacryl S-100 column with a 3901-fold increase in specific activity SDS-PAGE and zymogram activity staining showed a single band equal to molecular mass of 66 kDa Enzyme was found to be stable in presence of wide range of NaCl concentration with maximum activity found at 11% (w/v) of NaCl Enzyme showed remarkable stability towards laboratory surfactants detergents and oxidants Glucose maltose and maltotnose were the main end product of starch hydrolysis indicating it is a-amylase (c) 2010 Elsevier B V All rights reserved

引用

页码：52 / 58

页数：7

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