Oral Epithelial Cells are Activated via TRP Channels

被引:40
作者
Wang, B. [1 ]
Danjo, A. [1 ,2 ]
Kajiya, H. [3 ]
Okabe, K. [3 ]
Kido, M. A. [1 ]
机构
[1] Kyushu Univ, Grad Sch Dent Sci, Dept Oral Anat & Cell Biol, Higashi Ku, Fukuoka 8128582, Japan
[2] Saga Univ, Grad Sch Med, Dept Oral & Maxillofacial Surg, Saga, Japan
[3] Fukuoka Dent Coll, Dept Physiol Sci & Mol Biol, Fukuoka, Japan
基金
日本学术振兴会;
关键词
thermal; TRP channels; trigeminal; oral; RT-PCR; cell biology; multisensory perception; epithelia; neurophysiology; gingiva; keratinocyte(s); VANILLOID RECEPTOR EXPRESSION; KERATINOCYTES; SKIN; DIFFERENTIATION; VR1;
D O I
10.1177/0022034510385459
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Transient receptor potential (TRP) ion channels are critical contributors to the perception of various environmental stimuli. Although the oral cavity is the access point for various food types, the expression of TRP channels in oral mucosa remains unknown. We hypothesized that the oral epithelium itself may participate in sensing thermal, mechanical, and chemical conditions. The expression profiles of TRP channels exhibited regional differences among the buccal, palatal, and tongue epithelia. Changes in elevated intracellular Ca2+ concentration ([Ca2+](i)) in oral epithelial cells were found after stimulation of the TRP channels with capsaicin, camphor, 4 alpha-phorbol-12,13 didecanoate (4 alpha-PDD), 2-aminoethoxydiphenyl borate (2-APB), and menthol. These increases in Ca2+ appeared dependent on the TRP channels, because [Ca2+](i) suppression was observed after the addition of the TRPV channel antagonist ruthenium red. These results demonstrate that the oral epithelia express various TRP channels and may have functional roles in sensory activities, together with neurons.
引用
收藏
页码:163 / 167
页数:5
相关论文
共 26 条
[1]   The menthol receptor TRPM8 is the principal detector of environmental cold [J].
Bautista, Diana M. ;
Siemens, Jan ;
Glazer, Joshua M. ;
Tsuruda, Pamela R. ;
Basbaum, Allan I. ;
Stucky, Cheryl L. ;
Jordt, Sven-Eric ;
Julius, David .
NATURE, 2007, 448 (7150) :204-208
[2]   Role of the urothelium in urinary bladder dysfunction following spinal cord injury [J].
Birder, LA .
AUTONOMIC DYSFUNCTION AFTER SPINAL CORD INJURY, 2006, 152 :135-146
[3]   Vanilloid receptor expression suggests a sensory role for urinary bladder epithelial cells [J].
Birder, LA ;
Kanai, AJ ;
de Groat, WC ;
Kiss, S ;
Nealen, ML ;
Burke, NE ;
Dineley, KE ;
Watkins, S ;
Reynolds, IJ ;
Caterina, MJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (23) :13396-13401
[4]   Transient receptor potential ion channels as participants in thermosensation and thermoregulation [J].
Caterina, Michael J. .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2007, 292 (01) :R64-R76
[5]   Warm temperatures activate TRPV4 in mouse 308 keratinocytes [J].
Chung, MK ;
Lee, H ;
Caterina, MJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (34) :32037-32046
[6]   TRP channels as cellular sensors [J].
Clapham, DE .
NATURE, 2003, 426 (6966) :517-524
[7]  
Colton C. K., 2007, V179, P173
[8]   Immunoreactivity of VR1 on epidermal keratinocyte of human skin [J].
Denda, M ;
Fuziwara, S ;
Inoue, K ;
Denda, S ;
Akamatsu, H ;
Tomitaka, A ;
Matsunaga, K .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 285 (05) :1250-1252
[9]  
GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440
[10]   CALCIUM REGULATION OF GROWTH AND DIFFERENTIATION OF MOUSE EPIDERMAL-CELLS IN CULTURE [J].
HENNINGS, H ;
MICHAEL, D ;
CHENG, C ;
STEINERT, P ;
HOLBROOK, K ;
YUSPA, SH .
CELL, 1980, 19 (01) :245-254