Altered gene expression levels of IL-17/TRAF6/MAPK/USP25 axis and pro-inflammatory cytokine levels in lung tissue of obese ovalbumin-sensitized rats

被引：18

作者：

Aslani, Mohammad Reza ^{[1
,2
]}

Sharghi, Afshan ^{[3
]}

Boskabady, Mohammad Hossein ^{[2
,4
]}

Ghobadi, Hassan ^{[5
]}

Keyhanmanesh, Rana ^{[2
,6
]}

Alipour, Mohammad Reza ^{[6
]}

Ahmadi, Mahdi ^{[6
]}

Saadat, Saeideh ^{[2
,7
]}

Naghizadeh, Parya ^{[8
]}

机构：

[1] Ardabil Univ Med Sci, Fac Med, Dept Physiol, Ardebil, Iran

[2] Mashhad Univ Med Sci, Appl Biomed Res Ctr, Mashhad, Razavi Khorasan, Iran

[3] Ardabil Univ Med Sci, Fac Med, Dept Community Med, Ardebil, Iran

[4] Mashhad Univ Med Sci, Fac Med, Dept Physiol, Mashhad, Razavi Khorasan, Iran

[5] Ardabil Univ Med Sci, Fac Med, Internal Med Dept, Pulm Div, Ardebil, Iran

[6] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran

[7] Zahedan Univ Med Sci, Sch Med, Dept Physiol, Zahedan, Iran

[8] Ardabil Univ Med Sci, Fac Med, Ardebil, Iran

来源：

LIFE SCIENCES | 2022年 / 296卷

关键词：

Asthma; Obesity; IL-17A; IL-17F; USP25; MAPK; TRAF6; HIGH-FAT DIET; AIRWAY HYPERRESPONSIVENESS; KAPPA-B; TRACHEAL RESPONSIVENESS; MESSENGER-RNA; SMOOTH-MUSCLE; ASTHMA; IL-17A; CELLS; INTERLEUKIN-6;

D O I：

10.1016/j.lfs.2022.120425

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aims: The association between asthma and obesity has been shown but its accurate mechanism is unknown. In the current study, we sought to investigate the gene expression levels of IL-17/TRAF6/MAPK/USP25 axis and pro-inflammatory cytokine level (IL-6, IL-1 beta, and TNF-alpha) in obese Ovalbumin (OVA)-sensitized female and male Wistar rats lung tissue. Main methods: Animals in both males and females were divided into eight groups (four groups in each sex) based on diet and OVA-sensitization: normal diet, a normal diet with OVA-sensitization, high-fat diet (HFD), and OVA-sensitization with an HFD. Key findings: In both sexes, obese OVA-sensitized rats, the methacholine concentration-response curve shifted to the left and EC50 methacholine decreased. Increased pro-inflammatory cytokines as well as elevated IL-17/TRAF6/MAPK axis genes and decreased USP25 gene expression were identified in obese OVA-sensitized groups. Significance: The results indicate that in obese OVA-sensitized rats, the IL-17 axis were involved in the pathogenesis of the disease and can be considered as a therapeutic target in subjects with obesity-related asthma.

引用

页数：10