Silver nanoparticles with antimicrobial activities against Streptococcus mutans and their cytotoxic effect

被引：99

作者：

Alberto Perez-Diaz, Mario ^{[1
]}

Boegli, Laura ^{[2
]}

James, Garth ^{[2
]}

Velasquillo, Cristina ^{[3
]}

Sanchez-Sanchez, Roberto ^{[3
]}

Martinez-Martinez, Rita-Elizabeth ^{[4
]}

Alejandro Martinez-Castanon, Gabriel ^{[4
]}

Martinez-Gutierrez, Fidel ^{[1
]}

机构：

[1] UASLP, Fac Ciencias Quim, San Luis Potosi 78210, Slp, Mexico

[2] Montana State Univ, Ctr Biofilm Engn, Bozeman, MT 59717 USA

[3] Inst Nacl Rehabil, Lab Biotecnol, Mexico City, DF, Mexico

[4] Univ Autonoma San Luis Potosi, Fac Estomatol, San Luis Potosi, Mexico

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2015年 / 55卷

关键词：

Silver nanoparticles; Biofilm; Streptococcus mutans; CDC reactor; Anti-biofilm activity; Fibroblasts; ANTIBACTERIAL ACTIVITY; BACTERIAL; BIOFILMS; EFFICACY;

D O I：

10.1016/j.msec.2015.05.036

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Microbial resistance represents a challenge for the scientific community to develop new bioactive compounds. The goal of this research was to evaluate the antimicrobial activity of silver nanoparticles (AgNPs) against a clinical isolate of Streptococcus mutans, antibiofilm activity against mature S. mutans biofilms and the compatibility with human fibroblasts. The antimicrobial activity of AgNPs against the planktonic clinical isolate was size and concentration dependent, with smaller AgNPs having a lower minimum inhibitory concentration. A reduction of 23 log in the number of colony-forming units of S. mutans was observed when biofilms grown in a CDC reactor were exposed to 100 ppm of AgNPs of 9.5 +/- 1.1 nm. However, AgNPs at high concentrations (> 10 ppm) showed a cytotoxic effect upon human dermal fibroblasts. AgNPs effectively inhibited the growth of a planktonic S. mutatis clinical isolate and killed established S. mutans biofilms, which suggests that AgNPs could be used for prevention and treatment of dental caries. Further research and development are necessary to translate this technology into therapeutic and preventive strategies. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：360 / 366

页数：7

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