H3K36 methylation and DNA-binding both promote Ioc4 recruitment and Isw1b remodeler function

被引:5
作者
Li, Jian [1 ]
Bergmann, Lena [2 ]
de Almeida, Andreia Rafael [2 ]
Webb, Kimberly M. [3 ]
Gogol, Madelaine M. [4 ]
Voigt, Philipp [3 ,5 ]
Liu, Yingfang [1 ,6 ]
Liang, Huanhuan [1 ,7 ]
Smolle, Michaela M. [2 ,8 ]
机构
[1] Chinese Acad Sci, Inst Biophys, State Key Lab Biomacromol, 15 Datun Rd, Beijing 100101, Peoples R China
[2] Ludwig Maximilian Univ Munich, Med Fac, Biomed Ctr, Physiol Chem, Grosshaderner Str 9, D-82152 Martinsried, Germany
[3] Univ Edinburgh, Wellcome Ctr Cell Biol, Sch Biol Sci, Edinburgh EH9 3BF, Midlothian, Scotland
[4] Stowers Inst Med Res, 1000 E 50th St, Kansas City, MO 64110 USA
[5] Babraham Inst, Epigenet Programme, Cambridge CB22 3AT, England
[6] Sun Yat Sen Univ, Sch Med, Guangzhou 510275, Peoples R China
[7] Sun Yat Sen Univ, Pharmaceut Sci Shenzhen, Guangzhou 510275, Peoples R China
[8] Ludwig Maximilian Univ Munich, Med Fac, Biomed Ctr, BioPhys Core Facil, Grosshaderner Str 9, D-82152 Martinsried, Germany
基金
英国惠康基金; 欧洲研究理事会;
关键词
PWWP DOMAIN; HISTONE H3; STRUCTURAL BASIS; PHD FINGER; TRANSCRIPTION; RECOGNITION; CHD1; COMPLEX; FAMILY; GENES;
D O I
10.1093/nar/gkac077
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Isw1b chromatin-remodeling complex is specifically recruited to gene bodies to help retain pre-existing histones during transcription by RNA polymerase II. Recruitment is dependent on H3K36 methylation and the Isw1b subunit Ioc4, which contains an N-terminal PWWP domain. Here, we present the crystal structure of the Ioc4-PWWP domain, including a detailed functional characterization of the domain on its own as well as in the context of full-length Ioc4 and the Isw1b remodeler. The Ioc4-PWWP domain preferentially binds H3K36me3-containing nucleosomes. Its ability to bind DNA is required for nucleosome binding. It is also furthered by the unique insertion motif present in Ioc4-PWWP. The ability to bind H3K36me3 and DNA promotes the interaction of full-length Ioc4 with nucleosomes in vitro and they are necessary for its recruitment to gene bodies in vivo. Furthermore, a fully functional Ioc4-PWWP domain promotes efficient remodeling by Isw1b and the maintenance of ordered chromatin in vivo, thereby preventing the production of non-coding RNAs.
引用
收藏
页码:2549 / 2565
页数:17
相关论文
共 60 条
  • [1] JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila
    Albig, Christian
    Wang, Chao
    Dann, Geoffrey P.
    Wojcik, Felix
    Schauer, Tamas
    Krause, Silke
    Maenner, Sylvain
    Cai, Weili
    Li, Yeran
    Girton, Jack
    Muir, Tom W.
    Johansen, Jorgen
    Johansen, Kristen M.
    Becker, Peter B.
    Regnard, Catherine
    [J]. NATURE COMMUNICATIONS, 2019, 10 (1)
  • [2] The Chromatin Remodeler ISW1 Is a Quality Control Factor that Surveys Nuclear mRNP Biogenesis
    Babour, Anna
    Shen, Qingtang
    Dos-Santos, Julien
    Murray, Struan
    Gay, Alexandre
    Challal, Drice
    Fasken, Milo
    Palancade, Benoit
    Corbett, Anita
    Libri, Domenico
    Mellor, Jane
    Dargemont, Catherine
    [J]. CELL, 2016, 167 (05) : 1201 - +
  • [3] FACT facilitates transcription-dependent nucleosome alteration
    Belotserkovskaya, R
    Oh, S
    Bondarenko, VA
    Orphanides, G
    Studitsky, VM
    Reinberg, D
    [J]. SCIENCE, 2003, 301 (5636) : 1090 - 1093
  • [4] Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription
    Carrozza, MJ
    Li, B
    Florens, L
    Suganuma, T
    Swanson, SK
    Lee, KK
    Shia, WJ
    Anderson, S
    Yates, J
    Washburn, MP
    Workman, JL
    [J]. CELL, 2005, 123 (04) : 581 - 592
  • [5] A high-throughput ChIP-Seq for large-scale chromatin studies
    Christophe D Chabbert
    Sophie H Adjalley
    Bernd Klaus
    Emilie S Fritsch
    Ishaan Gupta
    Vicent Pelechano
    Lars M Steinmetz
    [J]. Molecular Systems Biology, 11 (1)
  • [6] The PWWP domain of Dnmt3a and Dnmt3b is required for directing DNA methylation to the major satellite repeats at pericentric heterochromatin
    Chen, TP
    Tsujimoto, N
    Li, E
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (20) : 9048 - 9058
  • [7] Heavy transcription of yeast genes correlates with differential loss of histone H2B relative to H4 and queued RNA polymerases
    Cole, Hope A.
    Ocampo, Josefina
    Iben, James R.
    Chereji, Razvan V.
    Clark, David J.
    [J]. NUCLEIC ACIDS RESEARCH, 2014, 42 (20) : 12512 - 12522
  • [8] Drosophila P75 safeguards oogenesis by preventing H3K9me2 spreading
    Dou, Kun
    Liu, Yanchao
    Zhang, Yingpei
    Wang, Chenhui
    Huang, Ying
    Zhang, Zz Zhao
    [J]. JOURNAL OF GENETICS AND GENOMICS, 2020, 47 (04) : 187 - 199
  • [9] Structural basis for high-affinity binding of LEDGF PWWP to mononucleosomes
    Eidahl, Jocelyn O.
    Crowe, Brandon L.
    North, Justin A.
    McKee, Christopher J.
    Shkriabai, Nikoloz
    Feng, Lei
    Plumb, Matthew
    Graham, Robert L.
    Gorelick, Robert J.
    Hess, Sonja
    Poirier, Michael G.
    Foster, Mark P.
    Kvaratskhelia, Mamuka
    [J]. NUCLEIC ACIDS RESEARCH, 2013, 41 (06) : 3924 - 3936
  • [10] IDENTIFICATION AND CHARACTERIZATION OF DROSOPHILA RELATIVES OF THE YEAST TRANSCRIPTIONAL ACTIVATOR SNF2/SWI2
    ELFRING, LK
    DEURING, R
    MCCALLUM, CM
    PETERSON, CL
    TAMKUN, JW
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (04) : 2225 - 2234