HsfA1d and HsfA1e Involved in the Transcriptional Regulation of HsfA2 Function as Key Regulators for the Hsf Signaling Network in Response to Environmental Stress

被引:183
作者
Nishizawa-Yokoi, Ayako [1 ]
Nosaka, Ryota [1 ]
Hayashi, Hideki [1 ]
Tainaka, Hitoshi [1 ]
Maruta, Takanori [1 ]
Tamoi, Masahiro [1 ]
Ikeda, Miho [2 ]
Ohme-Takagi, Masaru [2 ]
Yoshimura, Kazuya [3 ]
Yabuta, Yukinori [4 ]
Shigeoka, Shigeru [1 ]
机构
[1] Kinki Univ, Fac Agr, Dept Adv Biosci, Nara 6318505, Japan
[2] Natl Inst Adv Ind Sci & Technol, Gene Discovery Res Ctr, Tsukuba, Ibaraki 3058566, Japan
[3] Chubu Univ, Dept Food & Nutr Sci, Coll Biosci & Biotechnol, Aichi 4878501, Japan
[4] Tottori Univ, Fac Agr, Sch Agr Biol & Environm Sci, Tottori 6808553, Japan
基金
日本科学技术振兴机构;
关键词
Arabidopsis; CRES-T; Heat shock element; Hsf; Oxidative stress; Transcription factor; INTRACELLULAR-DISTRIBUTION; NUCLEAR IMPORT; ARABIDOPSIS; GENE; EXPRESSION; IDENTIFICATION; TOLERANCE; THERMOTOLERANCE; OVEREXPRESSION; ACCLIMATION;
D O I
10.1093/pcp/pcr045
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Heat shock transcription factor A2 (HsfA2) acts as a key component of the Hsf signaling network involved in cellular responses to various types of environmental stress. However, the mechanism governing the regulation of HsfA2 expression is still largely unknown. We demonstrated here that a heat shock element (HSE) cluster in the 5'-flanking region of the HsfA2 gene is involved in high light (HL)-inducible HsfA2 expression. Accordingly, to identify the Hsf regulating the expression of HsfA2, we analyzed the effect of loss-of-function mutations of class A Hsfs on the expression of HsfA2 in response to HL stress. Overexpression of an HsfA1d or HsfA1e chimeric repressor and double knockout of HsfA1d and HsfA1e Arabidopsis mutants (KO-HsfA1d/A1e) significantly suppressed the induction of HsfA2 expression in response to HL and heat shock (HS) stress. Transient reporter assays showed that HsfA1d and HsfA1e activate HsfA2 transcription through the HSEs in the 5'-flanking region of HsfA2. In the KO-HsfA1d/A1e mutants, 560 genes, including a number of stress-related genes and several Hsf genes, HsfA7a, HsfA7b, HsfB1 and HsfB2a, were down-regulated compared with those in the wild-type plants under HL stress. The PSII activity of KO-HsfA1d/A1e mutants decreased under HL stress, while the activity of wild-type plants remained high. Furthermore, double knockout of HsfA1d and HsfA1e impaired tolerance to HS stress. These findings indicated that HsfA1d and HsfA1e not only regulate HsfA2 expression but also function as key regulators of the Hsf signaling network in response to environmental stress.
引用
收藏
页码:933 / 945
页数:13
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