Decreased antioxidant defense during replicative aging of the yeast Saccharomyces cerevisiae studied using the 'baby machine' method

被引：8
|
作者：
Grzelak, A [1 ]
Skierski, J
Bartosz, G
机构：
[1] Univ Lodz, Dept Mol Biophys, Lodz, Poland
[2] Inst Drugs, Warsaw, Poland
[3] Pedag Univ Rzeszow, Dept Biochem & Cell Biol, Rzeszow, Poland
来源：
FEBS LETTERS | 2001年 / 492卷 / 1-2期
关键词：
aging; yeast; superoxide dismutase; glutathione; Saccharomyces cerevisiae;
D O I：
10.1016/S0014-5793(01)02244-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Replicatively senescent cells of Saccharomyces cerevisiae were obtained using the 'baby machine' method by immobilizing cells on CovaLinks(TM) NH2 plates and allowing them to divide while exchanging medium and removing daughter cells. Centrifugation in a Percoll density gradient was employed for further purification of replicatively old yeast cells. Comparison of senescent cells showing more than 20 bud stars with cells from early stationary culture demonstrated a significant reduction of total and reduced glutathione and decrease of superoxide dismutase activity during replicative aging of yeast cells. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
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页码：123 / +
页数：5
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