Specific Detection of Aquaporin-2 Using Plasmonic Tilted Fiber Grating Sensors

被引:37
作者
Han, Linzi [1 ]
Guo, Tuan [1 ]
Xie, Chen [2 ]
Xu, Peng [3 ]
Lao, Jiajie [1 ]
Zhang, Xuejun [1 ]
Xu, Jian [1 ]
Chen, Xiaoyong [1 ]
Huang, Yunyun [1 ]
Liang, Xing [3 ]
Mao, Wei [3 ]
Guan, Bai-Ou [1 ]
机构
[1] Jinan Univ, Inst Photon Technol, Guangdong Prov Key Lab Opt Fiber Sensing & Commun, Guangzhou 510632, Guangdong, Peoples R China
[2] Guangzhou Univ Chinese Med, Guangzhou 510006, Guangdong, Peoples R China
[3] Guangdong Prov Hosp Chinese Med, Guangzhou 510120, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
Biosensor; surface plasmon resonance; tilted fiber Bragg grating; PATHOPHYSIOLOGY; BIOSENSORS;
D O I
10.1109/JLT.2016.2645233
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
Aquaporin-2 (AQP2) has been identified as one of the most important biomarkers for nephrotic syndrome analysis. Here we propose and experimentally demonstrate a surface Plasmon resonance (SPR) reflective fiber-based biosensor for label-free specific detection of the urinary AQP2 with high resolution. The biosensor utilizes a tilted fiber Bragg grating with a 50 nm gold coating to excite SPR for the detection of refractive indices change at the fiber surface and the surroundings. The sensor works with very controllable cross-sensitivities because the core mode is temperature dependent but insensitive to surrounding refractive index. This label-free sensing system takes advantage of the specific functionalization of the sensor with AQP2 antibody, which has a high specificity to the target protein. The intensity variation of a selected plasmonic resonance caused by the specific capture of urinary AQP2 enables us to clearly differentiate between healthy and nephropathic samples in a few minutes, with the limit of detection up to 1.5 ng/ml.
引用
收藏
页码:3360 / 3365
页数:6
相关论文
共 38 条
[1]   Aquaporin water channels - from atomic structure to clinical medicine [J].
Agre, P ;
King, LS ;
Yasui, M ;
Guggino, WB ;
Ottersen, OP ;
Fujiyoshi, Y ;
Engel, A ;
Nielsen, S .
JOURNAL OF PHYSIOLOGY-LONDON, 2002, 542 (01) :3-16
[2]   High resolution grating-assisted surface plasmon resonance fiber optic aptasensor [J].
Albert, Jacques ;
Lepinay, Sandrine ;
Caucheteur, Christophe ;
DeRosa, Maria C. .
METHODS, 2013, 63 (03) :239-254
[3]   Optical fibre gratings as tools for chemical and biochemical sensing [J].
Baldini, F. ;
Brenci, M. ;
Chiavaioli, F. ;
Giannetti, A. ;
Trono, C. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2012, 402 (01) :109-116
[4]  
BERTANI T, 1982, LAB INVEST, V46, P16
[5]   Review of plasmonic fiber optic biochemical sensors: improving the limit of detection [J].
Caucheteur, Christophe ;
Guo, Tuan ;
Albert, Jacques .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2015, 407 (14) :3883-3897
[6]  
Deen PMT, 1998, CURR OPIN NEPHROL HY, V7, P37
[7]   Antiproteinuric effect of ciclosporin A in adriamycin nephropathy in rats [J].
Desassis, JF ;
Raats, CJI ;
Bakker, MAH ;
vandenBorn, J ;
Berden, JHM .
NEPHRON, 1997, 75 (03) :336-341
[8]  
Dunphy L.M., 2007, PRIMARY CARE ART SCI, V2nd
[9]   Sensitive optical biosensors for unlabeled targets: A review [J].
Fan, Xudong ;
White, Ian M. ;
Shopova, Siyka I. ;
Zhu, Hongying ;
Suter, Jonathan D. ;
Sun, Yuze .
ANALYTICA CHIMICA ACTA, 2008, 620 (1-2) :8-26
[10]  
Frokiaer J, 1998, AM J MED SCI, V316, P291