Protein analysis of moro blood orange pulp during storage at low temperatures

被引:19
作者
Carmona, L. [1 ]
Alquezar, B. [1 ,2 ]
Tarraga, S. [2 ]
Pena, L. [1 ,2 ]
机构
[1] Fundo Def Citricultura Fundecitrus, Ave Adhemar P Barros, Araraquara, SP, Brazil
[2] Univ Politecn Valencia, Inst Biol Mol & Celular Plantas, CSIC, Ingn Fausto Elio S-N, Valencia, Spain
基金
巴西圣保罗研究基金会;
关键词
Blood oranges; Anthocyanins; Cold storage; Purple juice; Proteome; ANTHOCYANIN BIOSYNTHESIS; FRUIT-QUALITY; COLD-STORAGE; ACCUMULATION; TRANSPORT; EXPRESSION; RESPONSES; VACUOLES; GENES;
D O I
10.1016/j.foodchem.2018.10.108
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A protein analysis in the pulp of Moro blood oranges (Citrus sinensis L. Osbeck) at the onset and after 30 days of storage at either 4 or 9 degrees C was performed. All differential proteins belonged to different functional classes (sugar, amino acid and secondary metabolism, defense, stress response, oxidative process, transport and cellular component biogenesis), displaying a differential accumulation in those Moro oranges kept at 9 versus 4 degrees C, and in those stored at 4 degrees C versus onset. Anthocyanin biosynthesis structural proteins chalcone synthases and flavonone 3-hydroxylase and different glutathione S-transferases related with their vacuolar transport were up-accumulated in fruits kept at 9 versus 4 degrees C and versus the onset. Proteins related with defense and oxidative stress displayed a similar pattern, concomitant with a higher anthocyanin content, denoting a possible role of defense and other stress response pathways in anthocyanin production/accumulation.
引用
收藏
页码:75 / 83
页数:9
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