Voltage-driven DNA translocations through a nanopore

被引：765

作者：

Meller, A ^{[1
]}

Nivon, L

Branton, D

机构：

[1] Rowland Inst Sci Inc, Cambridge, MA 02142 USA

[2] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[3] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

来源：

PHYSICAL REVIEW LETTERS | 2001年 / 86卷 / 15期

关键词：

D O I：

10.1103/PhysRevLett.86.3435

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We measure current blockade and time distributions for single-stranded DNA polymers during voltage-driven translocations through a single alpha -hemolysin pore. We use these data to determine the velocity of the polymers in the pore. Our measurements imply that, while polymers longer than the pore are translocated at a constant speed, the velocity of shorter polymers increases with decreasing length. This velocity is nonlinear with the applied field. Based on this data, we estimate the effective diffusion coefficient and the energy penalty for extending a molecule into the pore.

引用

页码：3435 / 3438

页数：4

共 15 条

[1] Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules [J].