DNA barcoding and mini-barcoding in authenticating processed animal-derived food: A case study involving the Chinese market

被引：35
作者：
Xing, Ran-Ran [1 ]
Hu, Ran-Ran [1 ,2 ]
Han, Jian-Xun [1 ,3 ]
Deng, Ting-Ting [1 ,2 ]
Chen, Ying [1 ]
机构：
[1] Chinese Acad Inspect & Quarantine, Agroprod Safety Res Ctr, Beijing 100176, Peoples R China
[2] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
[3] Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China
来源：
FOOD CHEMISTRY | 2020年 / 309卷
基金：
国家重点研发计划;
关键词：
DNA barcoding; Mini-barcoding; Species identification; Food authenticity; Animal-derived food; IDENTIFICATION; MEAT; PCR; FISH;
D O I：
10.1016/j.foodchem.2019.125653
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
This study used DNA barcoding and DNA mini-barcoding to test a variety of animal-derived food products sold in the Chinese market for potential mislabeling. Samples (52) including meat, poultry, and fish purchased from retail and online sources were examined. Regions of cytochrome C oxidase I (COI) gene (similar to 650 bp) and 16S rRNA (similar to 220 bp) were used as full- and mini-barcode markers, respectively. Approximately 94% (49 of 52) of the samples generated barcode sequences. The failure rate for full COI full-barcodes was 44%, but we obtained the 16S rRNA mini-barcode from 87% of the COI-failed cases. Overall, the survey revealed that 23% (12 of 52) of animal-derived products were mislabeled and, in most cases, contain undeclared species. Thus, regulatory measures and continuous monitoring for mislabeling of animal-derived products should be conducted.
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页数：7
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