Biocompatibility and biodistribution of functionalized carbon nano-onions (f-CNOs) in a vertebrate model

被引：56
作者：
d'Amora, Marta [1 ]
Rodio, Marina [2 ]
Bartelmess, Juergen [2 ,7 ]
Sancataldo, Giuseppe [1 ,3 ]
Brescia, Rosaria [4 ]
Zanacchi, Francesca Cella [1 ,5 ,8 ]
Diaspro, Alberto [1 ,5 ,6 ]
Giordani, Silvia [2 ]
机构：
[1] Ist Italiano Tecnol, Nanophys, Opt Nanoscopy, Via Morego 30, I-16163 Genoa, Italy
[2] Ist Italiano Tecnol, Nano Carbon Mat, Via Morego 30, I-16163 Genoa, Italy
[3] Univ Genoa, Dept Comp Sci Bioengn Robot & Syst Engn, Via Opera Pia 13, I-16145 Genoa, Italy
[4] Ist Italiano Tecnol, Nanochem Dept, Via Morego 30, I-16163 Genoa, Italy
[5] Ist Italiano Tecnol, NIC IIT, Via Morego 30, I-16163 Genoa, Italy
[6] Univ Genoa, Dept Phys, Via Dodecaneso 33, I-16145 Genoa, Italy
[7] BAM Fed Inst Mat Res & Testing, Div 1-9,Richard Willstaedter Str 11, D-12489 Berlin, Germany
[8] Inst Photon Sci ICFO, Av Carl Friedrich Gauss 3, Castelldefels 08860, Barcelona, Spain
来源：
SCIENTIFIC REPORTS | 2016年 / 6卷
关键词：
IN-VIVO; QUANTUM DOTS; ZEBRAFISH; NANOTUBES; NANOMATERIALS; FLUORESCENCE; GRAPHENE; NANOPARTICLES; EMBRYOS; TOXICITY;
D O I：
10.1038/srep33923
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Functionalized carbon nano-onions (f-CNOs) are of great interest as platforms for imaging, diagnostic and therapeutic applications due to their high cellular uptake and low cytotoxicity. To date, the toxicological effects of f-CNOs on vertebrates have not been reported. In this study, the possible biological impact of f-CNOs on zebrafish during development is investigated, evaluating different toxicity end-points such as the survival rate, hatching rate, and heart beat rate. Furthermore, a bio-distribution study of boron dipyrromethene (BODIPY) functionalized CNOs in zebrafish larvae is performed by utilizing inverted selective plane illumination microscopy (iSPIM), due to its intrinsic capability of allowing for fast 3D imaging. Our in vivo findings indicate that f-CNOs exhibit no toxicity, good biocompatibility (in the concentration range of 5-100 mu g mL(-1)) and a homogenous biodistribution in zebrafish larvae.
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页数：9
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