Large-scale production of functional human serum albumin from transgenic rice seeds

被引：176

作者：

He, Yang ^{[1
]}

Ning, Tingting ^{[1
]}

Xie, Tingting ^{[1
]}

Qiu, Qingchuan ^{[1
]}

Zhang, Liping ^{[1
]}

Sun, Yunfang ^{[1
]}

Jiang, Daiming ^{[1
]}

Fu, Kai ^{[1
]}

Yin, Fei ^{[2
]}

Zhang, Wenjing ^{[2
]}

Shen, Lang ^{[3
]}

Wang, Hui ^{[3
]}

Li, Jianjun ^{[4
]}

Lin, Qishan ^{[6
,7
]}

Sun, Yunxia ^{[5
]}

Li, Hongzhen ^{[5
]}

Zhu, Yingguo ^{[1
]}

Yang, Daichang ^{[1
]}

机构：

[1] Wuhan Univ, Engn Res Ctr Plant Biotechnol & Germplasm Utiliza, Minist Educ, State Key Lab Hybrid Rice,Coll Life Sci, Wuhan 430072, Peoples R China

[2] Healthgen Biotechnol Ltd Co, Wuhan 430074, Peoples R China

[3] Wuhan Univ, Basic Med Sch, Wuhan 430072, Peoples R China

[4] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

[5] Joinn Lab, Beijing 100176, Peoples R China

[6] SUNY Albany, Prote Spectrometry Serv, Rensselaer, NY 12144 USA

[7] SUNY Albany, Mass Spectrometry Serv, Rensselaer, NY 12144 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 47期

关键词：

crystal structure; recombinant human serum albumin; recombinant protein expression; rice endosperm; recombinant protein processing; RECOMBINANT PROTEINS; PICHIA-PASTORIS; GENE FLOW; EXPRESSION; SECRETION; ENDOSPERM; CIRRHOSIS; IMPROVES; ASCITES;

D O I：

10.1073/pnas.1109736108

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human serum albumin (HSA) is widely used in clinical and cell culture applications. Conventional production of HSA from human blood is limited by the availability of blood donation and the high risk of viral transmission from donors. Here, we report the production of Oryza sativa recombinant HSA (OsrHSA) from transgenic rice seeds. The level of OsrHSA reached 10.58% of the total soluble protein of the rice grain. Large-scale production of OsrHSA generated protein with a purity > 99% and a productivity rate of 2.75 g/kg brown rice. Physical and biochemical characterization of OsrHSA revealed it to be equivalent to plasma-derived HSA (pHSA). The efficiency of OsrHSA in promoting cell growth and treating liver cirrhosis in rats was similar to that of pHSA. Furthermore, OsrHSA displays similar in vitro and in vivo immunogenicity as pHSA. Our results suggest that a rice seed bioreactor produces cost-effective recombinant HSA that is safe and can help to satisfy an increasing worldwide demand for human serum albumin.

引用

页码：19078 / 19083

页数：6

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