Methyl-qPCR: a new method to investigate Epstein-Barr virus infection in post-transplant lymphoproliferative diseases

被引：6

作者：

Borde, Chloe ^{[1
]}

Quignon, Frederique ^{[2
]}

Amiel, Corinne ^{[3
]}

Gozlan, Joel ^{[4
]}

Marechal, Vincent ^{[1
]}

Brissot, Eolia ^{[5
,6
]}

机构：

[1] Sorbonne Univ, INSERM U938, Ctr Rech St Antoine, F-75012 Paris, France

[2] Sorbonne Univ, Inst Curie, CNRS UMR 144, Paris, France

[3] Hop Tenon, AP HP, Serv Virol, F-75020 Paris, France

[4] Sorbonne Univ, Hop St Antoine, AP HP, Serv Virol, F-75012 Paris, France

[5] Sorbonne Univ, Ctr Rech St Antoine CRSA, INSERM, F-75012 Paris, France

[6] Hop St Antoine, AP HP, Serv Hematol Clin & Therapie Cellulaire, F-75012 Paris, France

来源：

CLINICAL EPIGENETICS | 2022年 / 14卷 / 01期

关键词：

Epstein-Barr virus; DNA methylation; Epigenetics; Post-transplant lymphoproliferative disease; DISORDERS;

D O I：

10.1186/s13148-022-01255-1

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Epstein-Barr virus DNA viral load is used as a surrogate marker to start Rituximab in transplant recipients at risk of developing PTLD. However, an elevated EBV DNAemia does not discriminate lymphoproliferation and replication. We designed a new molecular assay (methyl-qPCR) to distinguish methylated versus unmethylated viral genomes. In blood, viral genomes were highly methylated in EBV primary infections, PTLD and 4/5 transplant recipients with high viral load. The only patient with under-methylated EBV genomes did not respond to rituximab. Methyl-qPCR is a convenient method to discriminate between latent and lytic EBV genomes and could be useful in treatment decisions.

引用

页数：5

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