Unraveling the Developmental and Genetic Mechanisms Underpinning Floral Architecture in Proteaceae

被引：10

作者：

Damerval, Catherine ^{[1
]}

Citerne, Helene ^{[1
]}

Silva, Natalia Conde e ^{[1
]}

Deveaux, Yves ^{[1
]}

Delannoy, Etienne ^{[2
]}

Joets, Johann ^{[1
]}

Simonnet, Franck ^{[1
,3
]}

Staedler, Yannick ^{[4
]}

Schonenberger, Jurg ^{[4
]}

Yansouni, Jennifer ^{[2
]}

Le Guilloux, Martine ^{[1
]}

Sauquet, Herve ^{[3
,5
]}

Nadot, Sophie ^{[3
]}

机构：

[1] Univ Paris Saclay, Univ Paris Sud, CNRS, INRA,AgroParisTech,GQE Le Moulon, Gif Sur Yvette, France

[2] Univ Paris Diderot, Paris Saclay, Paris Sud, Evry,CNRS,INRA,Inst Plant Sci Paris Saclay, Gif Sur Yvette, France

[3] Univ Paris Saclay, Univ Paris Sud, CNRS, Ecol Systemat Evolut,AgroParisTech, Orsay, France

[4] Univ Vienna, Dept Bot & Biodivers Res, Vienna, Austria

[5] Royal Bot Gardens & Domain Trust, Natl Herbarium New South Wales NSW, Sydney, NSW, Australia

来源：

FRONTIERS IN PLANT SCIENCE | 2019年 / 10卷

关键词：

Proteaceae; flower; development; floral symmetry; High Resolution X-Ray Computed Tomography; transcriptome; MADS-box genes; TCP genes; BHLH TRANSCRIPTION FACTOR; CARPEL DEVELOPMENT; TCP DOMAIN; ARABIDOPSIS; EVOLUTION; GROWTH; CLASSIFICATION; ZYGOMORPHY; SYMMETRY; GENOME;

D O I：

10.3389/fpls.2019.00018

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Proteaceae are a basal eudicot family with a highly conserved floral groundplan but which displays considerable variation in other aspects of floral and inflorescence morphology. Their morphological diversity and phylogenetic position make them good candidates for understanding the evolution of floral architecture, in particular the question of the homology of the undifferentiated perianth with the differentiated perianth of core eudicots, and the mechanisms underlying the repeated evolution of zygomorphy. In this paper, we combine a morphological approach to explore floral ontogenesis and a transcriptomic approach to access the genes involved in floral organ identity and development, focusing on Grevillea juniperina, a species from subfamily Grevilleoideae. We present developmental data for Grevillea juniperina and three additional species that differ in their floral symmetry using stereomicroscopy, SEM and High Resolution X-Ray Computed Tomography. We find that the adnation of stamens to tepals takes place at early developmental stages, and that the establishment of bilateral symmetry coincides with the asymmetrical growth of the single carpel. To set a framework for understanding the genetic basis of floral development in Proteaceae, we generated and annotated de novo a reference leaf/flower transcriptome from Grevillea juniperina. We found Grevillea homologs of all lineages of MADS-box genes involved in floral organ identity. Using Arabidopsis thaliana gene expression data as a reference, we found homologs of other genes involved in floral development in the transcriptome of G. juniperina. We also found at least 21 class I and class II TCP genes, a gene family involved in the regulation of growth processes, including floral symmetry. The expression patterns of a set of floral genes obtained from the transcriptome were characterized during floral development to assess their organ specificity and asymmetry of expression.

引用

页数：15

共 64 条

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