Bioaccumulation and Effects of CdTe/CdS Quantum Dots on Chlamydomonas reinhardtii - Nanoparticles or the Free Ions?

被引：105

作者：

Domingos, Rute F. ^{[1
,2
]}

Simon, Dana F. ^{[1
]}

Hauser, Charles ^{[3
]}

Wilkinson, Kevin J. ^{[1
]}

机构：

[1] Univ Montreal, Dept Chim, Montreal, PQ H3C 3J7, Canada

[2] Univ Tecn Lisbon, Ctr Quim Estrutural, Inst Super Tecn, P-1049001 Lisbon, Portugal

[3] St Edwards Univ, Bioinformat Program, Austin, TX 78704 USA

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 2011年 / 45卷 / 18期

基金：

加拿大自然科学与工程研究理事会;

关键词：

GREEN-ALGA; FOOD-CHAIN; CYTOTOXICITY; TOXICITY; CDSE; NANOCRYSTALS; NANOMATERIALS; ADSORPTION; METALS; GENOME;

D O I：

10.1021/es201193s

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In order to properly assess the environmental risk of engineered nanoparticles (ENP), it is necessary to determine their fate (including dissolution, aggregation, and bioaccumulation) under representative environmental conditions. CdTe/CdS quantum dots (QD), such as those used in medical imaging, are known to release Cd(2+) due (mainly) to the dissolution of their outer shell. In this study, Chlamydomonas reinhardtii was exposed to either a soluble Cd salt or QD at similar concentrations of total Cd. Free Cd concentrations were measured using the Absence of Gradients and Nernstian Equilibrium Stripping technique. QD dissolution increased with decreasing pH and with increasing QD concentration. When exposed to QD, bioaccumulation was largely accounted for by dissolved Cd. Nonetheless, QD were shown to be taken up by the cells and to provoke unique biological effects. Whole transcriptome screening using RNA-Seq analysis showed that the free Cd and the QD had distinctly different biological effects.

引用

页码：7664 / 7669

页数：6

共 40 条

[21] Cadmium Adsorption by Chlamydomonas reinhardtii and its Interaction with the Cell Wall Proteins [J].

Kola, Heliana ;

Laglera, Luis M. ;

Parthasarathy, Nalini ;

Wilkinson, Kevin J. .

ENVIRONMENTAL CHEMISTRY, 2004, 1 (03) :172-179

[22] Mapping short DNA sequencing reads and calling variants using mapping quality scores [J].

Li, Heng ;

Ruan, Jue ;

Durbin, Richard .

GENOME RESEARCH, 2008, 18 (11) :1851-1858

[23] The pc-1 phenotype of Chlamydomonas reinhardtii results from a deletion mutation in the nuclear gene for NADPH:protochlorophyllide oxidoreductase [J].

Li, JM ;

Timko, MP .

PLANT MOLECULAR BIOLOGY, 1996, 30 (01) :15-37

[24] Intracellular oxidative stress and cadmium ions release induce cytotoxicity of unmodified cadmium sulfide quantum dots [J].

Li, K. G. ;

Chen, J. T. ;

Bai, S. S. ;

Wen, X. ;

Song, S. Y. ;

Yu, Q. ;

Li, J. ;

Wang, Y. Q. .

TOXICOLOGY IN VITRO, 2009, 23 (06) :1007-1013

[25] Effects of Quantum Dots Adsorption on Algal Photosynthesis [J].

Lin, Sijie ;

Bhattacharya, Priyanka ;

Rajapakse, Nihal C. ;

Brune, David E. ;

Ke, Pu Chun .

JOURNAL OF PHYSICAL CHEMISTRY C, 2009, 113 (25) :10962-10966

[26] Unmodified cadmium telluride quantum dots induce reactive oxygen species formation leading to multiple organelle damage and cell death [J].

Lovric, J ;

Cho, SJ ;

Winnik, FM ;

Maysinger, D .

CHEMISTRY & BIOLOGY, 2005, 12 (11) :1227-1234

[27] Quantum Dot Weathering Results in Microbial Toxicity [J].

Mahendra, Shaily ;

Zhu, Huiguang ;

Colvin, Vicki L. ;

Alvarez, Pedro J. .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2008, 42 (24) :9424-9430

[28] Quantum dot bioconjugates for imaging, labelling and sensing [J].

Medintz, IL ;

Uyeda, HT ;

Goldman, ER ;

Mattoussi, H .

NATURE MATERIALS, 2005, 4 (06) :435-446

[29] The Chlamydomonas genome reveals the evolution of key animal and plant functions [J].

Merchant, Sabeeha S. ;

Prochnik, Simon E. ;

Vallon, Olivier ;

Harris, Elizabeth H. ;

Karpowicz, Steven J. ;

Witman, George B. ;

Terry, Astrid ;

Salamov, Asaf ;

Fritz-Laylin, Lillian K. ;

Marechal-Drouard, Laurence ;

Marshall, Wallace F. ;

Qu, Liang-Hu ;

Nelson, David R. ;

Sanderfoot, Anton A. ;

Spalding, Martin H. ;

Kapitonov, Vladimir V. ;

Ren, Qinghu ;

Ferris, Patrick ;

Lindquist, Erika ;

Shapiro, Harris ;

Lucas, Susan M. ;

Grimwood, Jane ;

Schmutz, Jeremy ;

Cardol, Pierre ;

Cerutti, Heriberto ;

Chanfreau, Guillaume ;

Chen, Chun-Long ;

Cognat, Valerie ;

Croft, Martin T. ;

Dent, Rachel ;

Dutcher, Susan ;

Fernandez, Emilio ;

Fukuzawa, Hideya ;

Gonzalez-Ballester, David ;

Gonzalez-Halphen, Diego ;

Hallmann, Armin ;

Hanikenne, Marc ;

Hippler, Michael ;

Inwood, William ;

Jabbari, Kamel ;

Kalanon, Ming ;

Kuras, Richard ;

Lefebvre, Paul A. ;

Lemaire, Stephane D. ;

Lobanov, Alexey V. ;

Lohr, Martin ;

Manuell, Andrea ;

Meier, Iris ;

Mets, Laurens ;

Mittag, Maria .

SCIENCE, 2007, 318 (5848) :245-251

[30] Quantum dots for live cells, in vivo imaging, and diagnostics [J].

Michalet, X ;

Pinaud, FF ;

Bentolila, LA ;

Tsay, JM ;

Doose, S ;

Li, JJ ;

Sundaresan, G ;

Wu, AM ;

Gambhir, SS ;

Weiss, S .

SCIENCE, 2005, 307 (5709) :538-544

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