Bioaccumulation and Effects of CdTe/CdS Quantum Dots on Chlamydomonas reinhardtii - Nanoparticles or the Free Ions?

被引:105
作者
Domingos, Rute F. [1 ,2 ]
Simon, Dana F. [1 ]
Hauser, Charles [3 ]
Wilkinson, Kevin J. [1 ]
机构
[1] Univ Montreal, Dept Chim, Montreal, PQ H3C 3J7, Canada
[2] Univ Tecn Lisbon, Ctr Quim Estrutural, Inst Super Tecn, P-1049001 Lisbon, Portugal
[3] St Edwards Univ, Bioinformat Program, Austin, TX 78704 USA
基金
加拿大自然科学与工程研究理事会;
关键词
GREEN-ALGA; FOOD-CHAIN; CYTOTOXICITY; TOXICITY; CDSE; NANOCRYSTALS; NANOMATERIALS; ADSORPTION; METALS; GENOME;
D O I
10.1021/es201193s
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
In order to properly assess the environmental risk of engineered nanoparticles (ENP), it is necessary to determine their fate (including dissolution, aggregation, and bioaccumulation) under representative environmental conditions. CdTe/CdS quantum dots (QD), such as those used in medical imaging, are known to release Cd(2+) due (mainly) to the dissolution of their outer shell. In this study, Chlamydomonas reinhardtii was exposed to either a soluble Cd salt or QD at similar concentrations of total Cd. Free Cd concentrations were measured using the Absence of Gradients and Nernstian Equilibrium Stripping technique. QD dissolution increased with decreasing pH and with increasing QD concentration. When exposed to QD, bioaccumulation was largely accounted for by dissolved Cd. Nonetheless, QD were shown to be taken up by the cells and to provoke unique biological effects. Whole transcriptome screening using RNA-Seq analysis showed that the free Cd and the QD had distinctly different biological effects.
引用
收藏
页码:7664 / 7669
页数:6
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