Generation of induced pluripotent stem cells (NIMHi001-A) from a Parkinson's disease patient of East Indian ethnicity carrying LRRK2 I1371V

被引：8

作者：

Datta, Indrani ^{[1
]}

Sowmithra ^{[1
]}

Jagtap, Soham ^{[1
]}

Potdar, Chandrakanta ^{[1
]}

Yadav, Ravi ^{[2
]}

Pal, Pramod ^{[2
]}

机构：

[1] Natl Inst Mental Hlth & Neurosci, Dept Biophys, Bengaluru 560029, Karnataka, India

[2] Natl Inst Mental Hlth & Neurosci, Dept Neurol, Bengaluru 560029, Karnataka, India

来源：

STEM CELL RESEARCH | 2020年 / 44卷

关键词：

Induced pluripotent stem cells; Parkinson's disease; Leucine Rich Repeat Kinase 2; I1371; variant; Indian ethnicity; FDOPA PET;

D O I：

10.1016/j.scr.2020.101768

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Mutations in the Leucine Repeat Rich Kinase-2 (LRRK2) gene have been reported in familial Parkinson's disease (PD) cases. We have generated induced pluripotent stem cells (iPSCs) using Sendai-virus reprogramming-method from peripheral blood mononuclear cells of PD-patient of East-Indian ethnicity carrying the I1371V mutation in LRRK2 gene. PD diagnosis was performed using Unified Parkinson's Disease rating scale (UPDRS) score and confirmed by [F-18]fluoro-L-dopa [F-DOPA] positron emission tomography (F-DOPA PET). The iPSC line was characterized for self-renewal and pluripotency. This cellular model will provide a valuable resource not only for drug-screening platform but also to understand the pathophysiology of this disease.

引用

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