High pressure processing inactivates human cytomegalovirus and hepatitis A virus while preserving macronutrients and native lactoferrin in human milk

被引：15

作者：

Pitino, Michael A. ^{[1
,2
]}

Unger, Sharon ^{[2
,3
,4
,5
]}

Gill, Alexander ^{[6
]}

McGeer, Allison J. ^{[7
,8
,9
]}

Doyen, Alain ^{[10
]}

Pouliot, Yves ^{[10
]}

Bazinet, Richard P. ^{[2
]}

Kothari, Akash ^{[1
]}

Mazzulli, Tony ^{[8
,9
]}

Stone, Debbie ^{[5
]}

O'Connor, Deborah L. ^{[1
,2
,4
,5
]}

机构：

[1] Hosp Sick Children, Translat Med Program, Toronto, ON, Canada

[2] Univ Toronto, Temerty Fac Med, Dept Nutr Sci, Toronto, ON, Canada

[3] Univ Toronto, Temerty Fac Med, Dept Pediat, Toronto, ON, Canada

[4] Sinai Hlth, Dept Pediat, Toronto, ON, Canada

[5] Sinai Hlth, Rogers Hixon Ontario Human Milk Bank, Toronto, ON, Canada

[6] Bur Microbial Hazards, Hlth Canada Sante Canada, Ottawa, ON, Canada

[7] Univ Toronto, Dalla Lana Sch Publ Hlth, Inst Hlth Policy Management & Evaluat, Toronto, ON, Canada

[8] Univ Toronto, Temerty Fac Med, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[9] Univ Hlth Network Sinai Hlth, Dept Microbiol, Toronto, ON, Canada

[10] Univ Laval, Ctr Rech STELA, Inst Nutr & Aliments Fonct, Dept Sci Aliments, Quebec City, PQ, Canada

来源：

INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES | 2022年 / 75卷

基金：

加拿大健康研究院;

关键词：

Donor human milk; Thermal pasteurization; Non -thermal processing; Viral infectivity; Plaque reduction assay; HUMAN NOROVIRUS; HEAT; PASTEURIZATION; PROTEINS; GROWTH;

D O I：

10.1016/j.ifset.2021.102891

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The effect of high pressure processing (HPP) compared to Holder pasteurization (HoP) (62.5 degrees C, 30 min), on the inactivation of cytomegalovirus (CMV) and hepatitis A virus (HAV) inoculated human milk pools (n = 10) and culture media (n = 3) was studied. Samples were retained as untreated controls, treated by HoP (62.5 degrees C, 30 min) or with one of six different HPP protocols (350 MPa, 500 MPa, 600 MPa for 8- or 10-min at 10 degrees C). Macronutrient concentration and lactoferrin were measured to confirm milk quality. Both HPP and HoP reduced CMV by 4.8-log PFU/mL and >0.9-log PFU/mL in culture medium and human milk, respectively. HoP reduced HAV by 3.4-log PFU/mL and 3.1-log PFU/mL in culture medium and human milk, respectively. HPP treatments of 500 or 600 MPa reduced HAV by >5.7-log PFU/mL and >4-log PFU/mL in culture medium and in human milk, respectively. Macronutrients (fat, total protein, carbohydrate) and energy composition was not affected by any treatment. Lactoferrin concentration decreased by 35% +/- 21% (SD) after HoP, but not HPP. Industrial relevance: This study confirms that HPP is effective in inactivating representative enveloped and nonenveloped viruses in human milk and reducing bacterial load, with no adverse effect on macronutrient and energy composition. For these reasons, evidence it reduces bacteria, and increased efficiency in which milk can be processed, HPP shows great promise in replacing HoP in human milk banking.

引用

页数：8

共 52 条

[1] Pasteurization of mother's own milk reduces fat absorption and growth in preterm infants [J].