Raman spectroscopy in investigations of mechanism of human serum albumin denaturation under action of sodium dodecyl sulfate

被引：24

作者：

Vlasova, I. M. ^{[1
]}

Polyansky, D. V. ^{[1
]}

Saletsky, A. M. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Phys, Moscow 119992, Russia

来源：

LASER PHYSICS LETTERS | 2007年 / 4卷 / 12期

关键词：

Raman spectroscopy; human serum albumin (HSA); denaturation; sodium dodecyl sulfate (SDS);

D O I：

10.1002/lapl.200710070

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The mechanism of denaturation of human serum albumin under action of an anionic detergent - sodium dodecyl sulfate (SDS) is investigated by Raman spectroscopy method. The quantitative contents of a-helical segments and random packing segments of polypeptide chain of albumin at different concentrations of SDS and at different pH values of solutions are determined. It is shown, that more intensive denaturation under action of SDS, consisting in disturbance of secondary and tertiary structure of albumin, takes place at pH values of solution, smaller the isoelectric point of protein.

引用

页码：890 / 894

页数：5

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