O-antigen chain-length distribution in Salmonella enterica serovar Enteritidis is regulated by oxygen availability

被引：4

作者：

Silva-Valenzuela, Cecilia A. ^{[1
,2
]}

Velasquez, Felipe ^{[1
]}

Penailillo, Johany ^{[1
]}

Garcias-Papayani, Hector ^{[1
]}

Fernandez, Paulina ^{[1
]}

Tobar, Pia ^{[1
]}

Contreras, Ines ^{[1
]}

Santiviago, Carlos A. ^{[1
]}

Alvarez, Sergio A. ^{[1
]}

机构：

[1] Univ Chile, Fac Ciencias Quim & Farmaceut, Dept Bioquim & Biol Mol, Santiago, Chile

[2] Tufts Univ, Dept Mol Biol & Microbiol, Boston, MA 02111 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 477卷 / 04期

关键词：

Salmonella; Lipopolysaccharide; O antigen; Anaerobiosis; ArcA; Fnr; ESCHERICHIA-COLI; CHROMOSOMAL GENES; SERUM RESISTANCE; EXPRESSION; FNR; LIPOPOLYSACCHARIDE; TYPHIMURIUM; GROWTH; ARCA; TRANSCRIPTION;

D O I：

10.1016/j.bbrc.2016.06.074

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipopolysaccharide (LPS) consists of three covalently linked domains: the lipid A, the core region and the O antigen (OAg), consisting of repeats of an oligosaccharide. Salmonella enterica serovar Enteritidis (S. Enteritidis) produces a LPS with two OAg preferred chain lengths: a long (L)-OAg controlled by Wzz(SE) and a very long (VL)-OAg controlled by Wzz(fepE). In this work, we show that OAg produced by S. Enteritidis grown in E minimal medium also presented two preferred chain-lengths. However, a simultaneous and opposing change in the production of L-OAg and VL-OAg was observed in response to oxygen availability. Biochemical and genetics analyses indicate that this process is regulated by transcriptional factors Fnr and ArcA by means of controlling the transcription of genes encoding Wzz(SE) and Wzz(fepE) in response to oxygen availability. Thus, our results revealed a sophisticated regulatory mechanism involved in the adaptation of S. Enteritidis to one of the main environmental cues faced by this pathogen during infection. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：563 / 567

页数：5

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