A Novel Method for Transmission Electron Microscopy Study of Cytoplasmic Fragments From Preimplantation Human Embryos

被引：5

作者：

Halvaei, Iman ^{[1
]}

Khalili, Mohammad Ali ^{[1
]}

Nottola, Stefania A. ^{[2
]}

机构：

[1] Shahid Sadoughi Univ Med Sci, Res & Clin Ctr Infertil, Yazd, Iran

[2] Univ Roma La Sapienza, Dept Anat Histol Forens Med & Orthopaed, Piazzale Aldo Moro 5, I-00185 Rome, Italy

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2016年 / 79卷 / 06期

关键词：

transmission electron microscopy; embryo; fragmentation; empty zona pellucida; IN-VITRO; QUALITY; OOCYTES; MARKERS;

D O I：

10.1002/jemt.22657

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Transmission electron microscopy (TEM) is the main tool for exploring the intracellular damage and organelle distribution. The cause of producing embryo cytoplsamic fragmentation is not completely understood. Since the fragments have detrimental effects on embryo development, the ultrastructural analysis of fragments may play an important role in fragmentation etiology and in embryo development as well. There are no studies regarding the ultrastructure of fragments in transferable embryos, because the preparation for TEM is not vital and embryos are discarded inevitably. This study aims to introduce a new method for ultrastructural evaluation of fragments without damaging the human cleaving embryos. (C) 2016 Wiley Periodicals, Inc.

引用

页码：459 / 462

页数：4

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