Assessment of Lactotroph Axis Functionality in Mice: Longitudinal Monitoring of PRL Secretion by Ultrasensitive-ELISA

被引:47
作者
Guillou, Anne [1 ,2 ,3 ,4 ,5 ,6 ]
Romano, Nicola [1 ,2 ,3 ,4 ,5 ,6 ]
Steyn, Frederik [7 ]
Abitbol, Karine [1 ,2 ,3 ,4 ,5 ,6 ]
Le Tissier, Paul [8 ]
Bonnefont, Xavier [1 ,2 ,3 ,4 ,5 ,6 ]
Chen, Chen [7 ]
Mollard, Patrice [1 ,2 ,3 ,4 ,5 ,6 ]
Martin, Agnes O. [1 ,2 ,3 ,4 ,5 ,6 ]
机构
[1] Univ Montpellier I, UMR 5203, F-34000 Montpellier, France
[2] Univ Montpellier I, CNRS, Inst Genom Fonct, INSERM, F-34000 Montpellier, France
[3] Univ Montpellier I, U661, F-34000 Montpellier, France
[4] Univ Montpellier 2, UMR 5203, F-34000 Montpellier, France
[5] Univ Montpellier 2, CNRS, Inst Genom Fonct, INSERM, F-34000 Montpellier, France
[6] Univ Montpellier 2, U661, F-34000 Montpellier, France
[7] Univ Queensland, Fac Med & Biomed Sci, Sch Biomed Sci, St Lucia, Qld 4072, Australia
[8] Univ Edinburgh, Ctr Integrat Physiol, Edinburgh EH8 9XD, Midlothian, Scotland
基金
英国医学研究理事会;
关键词
PROLACTIN SECRETION; MATERNAL-BEHAVIOR; GROWTH-HORMONE; HYPOTHALAMIC DOPAMINE; LUTEINIZING-HORMONE; LACTATING RATS; ESTROUS-CYCLE; FEMALE RATS; MOUSE; PLASMA;
D O I
10.1210/en.2014-1571
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The pattern of prolactin (PRL) secretion depends on the physiological state. Due to insufficient detection sensitivity of existing assays, the precise description of these patterns in mice is lacking. We described an ultrasensitive ELISA assay that can detect mouse PRL in small fractions of whole blood, allowing longitudinal studies of PRL secretion profiles in freely moving mice. Over a 24-hour period, males displayed no oscillation in PRL levels, whereas virgin and lactating females showed large pulses. Peaks of PRL secretion reached 30-40 ng/mL in lactating female mice and rarely exceeded 10 ng/mL in virgin females. These pulses of PRL in lactating females were associated with suckling. The return of pups after an experimental 12-hour weaning induced a pulse of PRL release, reaching 100 ng/mL. This approach also enabled us to assess the inhibitory tone from hypothalamic dopamine neurons on PRL secretion. We used a dopamine D2 receptor antagonist to relieve pituitary lactotrophs from the tuberoinfundibular dopaminergic inhibitory tone and demonstrate a D2-induced PRL rise that can be used to evaluate both the secretory capacity of lactotrophs and the magnitude of the inhibitory tone on pituitary PRL release. We demonstrate that, although lactotroph function is altered to enhance chronic PRL output, their secretory response to acute stimulus is not modified during lactation and that chronic hyperprolactinemia is linked to a lower inhibitory tone. The combination of a sensitive PRL ELISA and administration of D2 receptor antagonist provide a unique opportunity to investigate the function and plasticity of the lactotroph axis in freely moving mice.
引用
收藏
页码:1924 / 1930
页数:7
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