Desensitization of the cannabinoid CBI receptor is mediated by the interaction with arrestin. In this study, we report the structural changes of a synthetic diphosphorylated peptide corresponding to residues 419-439 of the CBI C-terminus upon binding to arrestin-2. This segment is pivotal to the desensitization of CBI. Using high-resolution proton NMR, we observe two helical segments in the bound peptide that are separated by the presence a glycine residue. The binding we observe is with a diphoshorylated peptide, whereas a previous study reported binding of a highly phosphorylated rhodopsin fragment to visual arrestin. The arrestin bound conformations of the peptides are compared. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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Duke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USADuke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USA
Gainetdinov, RR
Premont, RT
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机构:Duke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USA
Premont, RT
Bohn, LM
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机构:Duke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USA
Bohn, LM
Lefkowitz, RJ
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机构:Duke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USA
Lefkowitz, RJ
Caron, MG
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机构:Duke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USA
机构:
Duke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USADuke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USA
Gainetdinov, RR
Premont, RT
论文数: 0引用数: 0
h-index: 0
机构:Duke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USA
Premont, RT
Bohn, LM
论文数: 0引用数: 0
h-index: 0
机构:Duke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USA
Bohn, LM
Lefkowitz, RJ
论文数: 0引用数: 0
h-index: 0
机构:Duke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USA
Lefkowitz, RJ
Caron, MG
论文数: 0引用数: 0
h-index: 0
机构:Duke Univ, Med Ctr, Howard Hughes Med Inst Labs, Dept Cell Biol, Durham, NC 27710 USA