Fluorescence of the DNA double helices (dAdT)n•(dAdT)n studied by femtosecond spectroscopy

被引：47

作者：

Onidas, Delphine ^{[1
]}

Gustavsson, Thomas ^{[1
]}

Lazzarotto, Elodie ^{[1
]}

Markovitsi, Dimitra ^{[1
]}

机构：

[1] CEA Saclay, CNRS, URA 2453, SPAM,DSM,CEA,Lab Francis Perrin, F-91191 Gif Sur Yvette, France

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2007年 / 9卷 / 37期

关键词：

D O I：

10.1039/b707882j

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Polymeric and oligomeric DNA helices, poly(dAdT) . poly(dAdT) and (dAdT)(10) . (dAdT)(10), composed of 200-400 and 20 adenine-thymine base pairs, respectively, are studied by fluorescence upconversion. Fluorescence decays, anisotropy decays and time-resolved spectra, obtained for this alternating base sequence, are compared with those determined previously for the homopolymeric sequence (dA)(n) . (dT)(n). It is shown that identical fluorescence decays may correspond to quite different anisotropy decays and vice versa, both varying with the emission wavelength, the base sequence and the duplex size. Our observations cannot be explained in terms of monomer and excimer emission exclusively, as concluded in the past on the basis of steady-state measurements. Excitons also contribute to the fluorescence. These are rapidly trapped by excimers, characterized by long-lived weak emission.

引用

页码：5143 / 5148

页数：6

共 25 条

[1] Energy transfer in unsymmetrical phenylene ethynylene dendrimers [J].

Atas, E ;

Peng, ZH ;

Kleiman, VD .

JOURNAL OF PHYSICAL CHEMISTRY B, 2005, 109 (28) :13553-13560

[2] Influence of conformational dynamics on the exciton states of DNA oligomers [J].

Bouvier, B ;

Dognon, JP ;

Lavery, R ;

Markovitsi, D ;

Millié, P ;

Onidas, D ;

Zakrzewska, K .

JOURNAL OF PHYSICAL CHEMISTRY B, 2003, 107 (48) :13512-13522

[3] Dipolar coupling between electronic transitions of the DNA bases and its relevance to exciton states in double helices [J].

Bouvier, B ;

Gustavsson, T ;

Markovitsi, D ;

Millié, P .

CHEMICAL PHYSICS, 2002, 275 (1-3) :75-92

[4] Calculations and characterization of the electronic spectra of DNA bases based on ab initio MP2 geometries of different tautomeric forms [J].

Broo, A ;

Holmen, A .

JOURNAL OF PHYSICAL CHEMISTRY A, 1997, 101 (19) :3589-3600

[5] Electronic energy delocalization and dissipation in single- and double-stranded DNA [J].

Buchvarov, Ivan ;

Wang, Qiang ;

Raytchev, Milen ;

Trifonov, Anton ;

Fiebig, Torsten .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (12) :4794-4797

[6] Base stacking controls excited-state dynamics in A-T DNA [J].

Crespo-Hernández, CE ;

Cohen, B ;

Kohler, B .

NATURE, 2005, 436 (7054) :1141-1144

[7] The role of UV-B light in skin carcinogenesis through the analysis of p53 mutations in squamous cell carcinomas of hairless mice [J].

Dumaz, N ;

vanKranen, HJ ;

deVries, A ;

Berg, RJW ;

Wester, PW ;

vanKreijl, CF ;

Sarasin, A ;

DayaGrosjean, L ;

deGruijl, FR .

CARCINOGENESIS, 1997, 18 (05) :897-904

[8] Exciton states of dynamic DNA double helices:: Alternating dCdG sequences [J].

Emanuele, E ;

Zakrzewska, K ;

Markovitsi, D ;

Lavery, R ;

Millié, P .

JOURNAL OF PHYSICAL CHEMISTRY B, 2005, 109 (33) :16109-16118

[9] UV spectra and excitation delocalization in DNA:: Influence of the spectral width [J].

Emanuele, E ;

Markovitsi, D ;

Millié, P ;

Zakrzewska, K .

CHEMPHYSCHEM, 2005, 6 (07) :1387-1392

[10] EXCITED-STATE PROPERTIES OF THE ALTERNATING POLYNUCLEOTIDE POLY(DA-DT).POLY(DA-DT) [J].

GE, G ;

GEORGHIOU, S .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1991, 54 (02) :301-305

← 1 2 3 →