Proteomics of human cancer tissues and cells

被引：11

作者：

Cecconi, Daniela ^{[1
]}

Zamo, Alberto ^{[2
]}

机构：

[1] Univ Verona, Dept Biotechnol, Mass Spectrometry & Prote Lab, I-37134 Verona, Italy

[2] Univ Verona, Policlin GB Rossi, Sect Anat Pathol, Dept Pathol & Diagnost, I-37134 Verona, Italy

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2011年 / 30卷 / 02期

关键词：

Array-based proteomic technology; Biomarker; Candidate biomarker; Cancer cell; Cancer research; Cancer tissue; Mass spectrometry; Protein profiling; Proteomic technology; Shotgun proteomics; IMAGING MASS-SPECTROMETRY; PLASMA-MEMBRANE PROTEINS; GROWTH-FACTOR RECEPTOR; FREE QUANTITATIVE PROTEOMICS; PARAFFIN-EMBEDDED TISSUES; HUMAN COLON-CANCER; BREAST-CANCER; LABEL-FREE; ABSOLUTE QUANTIFICATION; LIQUID-CHROMATOGRAPHY;

D O I：

10.1016/j.trac.2010.10.016

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Proteomic analysis of cancer tissues and cells provides valuable information to identify promising targets for cancer diagnosis, prognosis and therapy. Novel strategies have emerged to optimize the workflow of tissue procurement, and tissue and cell selection, and to improve protocols for the extraction of protein from fresh, frozen and paraffin-embedded tissue. Moreover, in the context of advanced approaches to proteomics, mass spectrometry and array-based technologies strongly contribute to protein profiling of cancer tissues and cells. The focus of this review is the methods by which all the steps of a proteomic investigation on human-cancer tissue (from choice of the experimental model to validation of candidate biomarkers) should be performed, paying particular attention to recently developed strategies. The review also presents an overview of the most recent high-throughput proteomic studies in cancer research. (c) 2010 Elsevier Ltd. All rights reserved.

引用

页码：346 / 359

页数：14

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