Oxidation of glucose to gluconic acid by glucose oxidase in a membrane Bioreactor

被引：23

作者：

Tomotani, EJ ^{[1
]}

Das Neves, LCM ^{[1
]}

Vitolo, M ^{[1
]}

机构：

[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Pharmaceut Technol & Biochem, BR-05508900 Sao Paulo, Brazil

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2005年 / 121卷 / 1-3期

关键词：

glucose oxidase; membrane bioreactor; gluconic acid; stability; feeding rate;

D O I：

10.1385/ABAB:121:1-3:0149

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucose oxidase (GO) (EC 1.1.3.4) was used as catalyst for oxidizing glucose into gluconic acid utilizing a 10-mL Bioengmeermg Enzyme Membrane Reactor (R) or a 400-ml, Millipore Stirred Ultrafiltration Cell (MSUC) coupled with a Millipore UF membrane (cutoff of 100 kDa) and operated for 12 h under an agitation of 100 rpm, pH 5.5, and 30 degrees C. The effect of feeding rate (0.10, 0.15, or 0.20 min(-1)), glucose (2.5 or 5.0 mM), and GO (1.0 or 2.0 mg/mL) concentrations on the catalysis were studied. A yield of about 75% was attained when the MSUC filled with 1.0 mg/mL of GO was fed with 2.5 mM glucose solution at a rate of 0.15 min(-1).

引用

页码：149 / 162

页数：14

共 21 条

[1] A kinetic study on crystallization of calcium gluconate in external loop airlift column and stirred tank for an immobilized glucose oxidase reaction with crystallization [J].