Flow cytometric and functional analyses of central nervous system-infiltrating cells in SJL/J mice with Theiler's virus-induced demyelinating disease - Evidence for a CD4(+) T cell-mediated pathology

Theiler's murine encephalomyelitis viruses (TMEVs) are endemic enteric pathogens of mice that cause immune-mediated, chronic, progressive, central nervous system (CNS) demyelinating disease in susceptible strains. Analysis of T cell phenotype and functional state from TMEV/-infected SJL/J mice by flow cytometry reveals that 13.5 to 25% of the CD4(+) T cells in the CNS express high affinity IL-2R, a marker of recent T cell activation, whereas splenic levels of CD4(+)IL-2R(+) T cells generally range between 2 and 8.5%. In contrast, very few CD8(+) T cells (<1-2%) from either site express IL-2R. From days 20 to 119 postinfection, the percentage of CD4(+)IL-2R(+) T cells increases gradually in the CNS, but varies little in the spleen. CD4(+) T cells isolated from the spinal cord of infected mice proliferate in vitro in response to viral Ag. Similar T cell phenotypes were found in experimental autoimmune encephalomyelitis, an established model of CD4(+) T cell-mediated demyelination. In addition, most CD4(+) and CD8(+) T cells in CNS isolates from TMEV-infected mice are CD44(+), indicating that prior activation may be required to traffic through and/or be retained in the CNS. finally, TCR VP region usage as well as IL-2R expression by individual VP region subsets are heterogeneous in both the CNS and spleen. These results are consistent with a model in which a polyclonal population of TMEV-specific, CD4(+) Th1 cells plays a major effector role in the demyelinating process.