Nanobiosensing Platforms for Real-Time and Non-Invasive Monitoring of Stem Cell Pluripotency and Differentiation

被引：18

作者：

Suhito, Intan Rosalina ^{[1
]}

Angeline, Novi ^{[1
]}

Choo, Sung-Sik ^{[1
]}

Woo, Ho Young ^{[1
]}

Paik, Taejong ^{[1
]}

Lee, Taek ^{[2
]}

Kim, Tae-Hyung ^{[1
,3
]}

机构：

[1] Chung Ang Univ, Sch Integrat Engn, Seoul 06974, South Korea

[2] Kwangwoon Univ, Dept Chem Engn, 20 Kwangwoon Ro, Seoul 0189, South Korea

[3] Chung Ang Univ, Inst Interdisciplinary Convergence Res, Integrat Res Ctr 2 Dimens Funct Mat, Seoul 06974, South Korea

来源：

SENSORS | 2018年 / 18卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

stem cells; differentiation; pluripotency; electrochemical detection; Raman spectroscopy; MESENCHYMAL STROMAL CELLS; RAMAN-SPECTROSCOPY; ELECTROCHEMICAL DETECTION; IN-VITRO; OSTEOGENIC DIFFERENTIATION; GOLD NANOPARTICLES; ARRAYS; FABRICATION; INDUCTION; ORGANS;

D O I：

10.3390/s18092755

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Breakthroughs in the biomedical and regenerative therapy fields have led to the influential ability of stem cells to differentiate into specific types of cells that enable the replacement of injured tissues/organs in the human body. Non-destructive identification of stem cell differentiation is highly necessary to avoid losses of differentiated cells, because most of the techniques generally used as confirmation tools for the successful differentiation of stem cells can result in valuable cells becoming irrecoverable. Regarding this issue, recent studies reported that both Raman spectroscopy and electrochemical sensing possess excellent characteristics for monitoring the behavior of stem cells, including differentiation. In this review, we focus on numerous studies that have investigated the detection of stem cell pluripotency and differentiation in non-invasive and non-destructive manner, mainly by using the Raman and electrochemical methods. Through this review, we present information that could provide scientific or technical motivation to employ or further develop these two techniques for stem cell research and its application.

引用

页数：17

共 72 条

[1] In vitro differentiation of neural stem cells derived from human olfactory bulb into dopaminergic-like neurons [J].