Predicting Antibiotic Resistance in Gram-Negative Bacilli from Resistance Genes

被引：0

作者：

Walker, G. Terrance ^{[1
]}

Quan, Julia ^{[1
]}

Higgins, Stephen G. ^{[1
]}

Toraskar, Nikhil ^{[1
]}

Chang, Weizhong ^{[1
]}

Saeed, Alexander ^{[1
]}

Sapiro, Vadim ^{[1
]}

Pitzer, Kelsey ^{[1
]}

Whitfield, Natalie ^{[1
,6
]}

Lopansri, Bert K. ^{[2
,3
]}

Motyl, Mary ^{[4
]}

Sahm, Daniel ^{[5
]}

机构：

[1] OpGen Inc, Gaithersburg, MD 20878 USA

[2] Intermt Med Ctr, Murray, UT USA

[3] Univ Utah, Salt Lake City, UT USA

[4] Merck & Co Inc, Whitehouse Stn, NJ USA

[5] IHMA Inc, Schaumburg, MA USA

[6] GenMark Diagnost Inc, Carlsbad, CA USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2019年 / 63卷 / 04期

关键词：

PCR; antibiotic resistance; resistance genes;

D O I：

10.1128/AAC.02462-18

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a rapid high-throughput PCR test and evaluated highly antibiotic-resistant clinical isolates of Escherichia coli (n = 2,919), Klebsiella pneumoniae (n = 1,974), Proteus mirabilis (n = 1,150), and Pseudomonas aeruginosa (n = 1,484) for several antibiotic resistance genes for comparison with pheno-typic resistance across penicillins, cephalosporins, carbapenems, aminoglycosides, trimethoprim-sulfamethoxazole, fluoroquinolones, and macrolides. The isolates originated from hospitals in North America (34%), Europe (23%), Asia (13%), South America (12%), Africa (7%), or Oceania (1%) or were of unknown origin (9%). We developed statistical methods to predict phenotypic resistance from resistance genes for 49 antibiotic-organism combinations, including gentamicin, tobramycin, ciprofloxacin, levofloxacin, trimethoprim-sulfamethoxazole, ertapenem, imipenem, cefazolin, cefepime, cefotaxime, ceftazidime, ceftriaxone, ampicillin, and aztreonam. Average positive predictive values for genotypic prediction of phenotypic resistance were 91% for E. coli, 93% for K. pneumoniae, 87% for P. mirabilis, and 92% for P. aeruginosa across the various antibiotics for this highly resistant cohort of bacterial isolates.

引用

页数：9

共 13 条

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