Chemical characterization of acidic oligosaccharides in milk of the red kangaroo (Macropus rufus)

被引：16
作者：
Anraku, Tatsuro [1 ]
Fukuda, Kenji [1 ]
Saito, Tadao [2 ]
Messer, Michael [3 ]
Urashima, Tadasu [1 ]
机构：
[1] Obihiro Univ Agr & Vet Med, Grad Sch Food Hyg, Obihiro, Hokkaido 0808555, Japan
[2] Tohoku Univ, Grad Sch Agr, Sendai, Miyagi 9818555, Japan
[3] Univ Sydney, Sch Mol & Microbial Biosci, Sydney, NSW 2006, Australia
来源：
GLYCOCONJUGATE JOURNAL | 2012年 / 29卷 / 2-3期
关键词：
Red kangaroo; Milk oligosaccharides; Sialyl oligosaccharide; Oligosaccharide sulfate; Galactosyllactose; Lacto-N-novopentaose I; Macrupus rufus; 3 NEUTRAL OLIGOSACCHARIDES; MARSUPIAL-MILK; LACTOSE SULFATE; TAMMAR WALLABY; STRUCTURAL DETERMINATION; ECHIDNA MILK; COLOSTRUM; EUGENII; CHROMATOGRAPHY; TRISACCHARIDE;
D O I：
10.1007/s10719-012-9372-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In the milk of marsupials, oligosaccharides usually predominate over lactose during early to mid lactation. Studies have shown that tammar wallaby milk contains a major series of neutral galactosyllactose oligosaccharides ranging in size from tri- to at least octasaccharides, as well as beta(1-6) linked N-acetylglucosamine-containing oligosaccharides as a minor series. In this study, acidic oligosaccharides were purified from red kangaroo milk and characterized by H-1-nuclear magnetic resonance spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, to be as follows: Neu5Ac(alpha 2-3)Gal(beta 1-4)Glc (3'-SL), Neu5Ac(alpha 2-3)Gal(beta 1-3)Gal(beta 1-4)Glc (sialyl 3'-galactosyllactose), Neu5Ac(alpha 2-3)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-4)Glc, Neu5Ac(alpha 2-3)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-4)Glc, Neu5Ac(alpha 2-3)Gal(beta 1-3)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc (sialyl lacto-N-novopentaose a), Gal(beta 1-3)[Neu5Ac(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc (sialyl lacto-N-novopentaose b), Neu5Ac(alpha 2-3)Gal(beta 1-3)Gal(beta 1-3)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc, Gal(beta 1-3)(-3-O-sulfate)Gal(beta 1-3)Gal(beta 1-4)Glc, Gal(beta 1-3)(-3-O-sulfate)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-4)Glc, Gal(beta 1-3)(-3-O-sulfate)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-3)Gal(beta 1-4)Glc, Gal(beta 1-3)(-3-O-sulfate)Gal(beta 1-3)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc, Gal(beta 1-3)(-3-O-sulfate)Gal(beta 1-3)Gal(beta 1-3)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc. These acidic oligosaccharides were shown to be sialylated or sulfated in the non-reducing ends to the major linear and the minor branched series of neutral oligosaccharides of tammar wallaby milk.
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页码：147 / 156
页数：10
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