Designation of enzyme activity of glycine-N-acyltransferase family genes and depression of glycine-N-acyltransferase in human hepatocellular carcinoma

被引：40

作者：

Matsuo, Moe ^{[1
]}

Terai, Kensuke ^{[2
]}

Kameda, Noriaki ^{[2
]}

Matsumoto, Aya ^{[1
]}

Kurokawa, Yumiko ^{[1
]}

Funase, Yuichi ^{[1
]}

Nishikawa, Kazuko ^{[3
]}

Sugaya, Naoki ^{[1
]}

Hiruta, Nobuyuki ^{[2
]}

Kishimoto, Toshihiko ^{[1
,4
]}

机构：

[1] Toho Univ, Dept Biomol Sci, Fac Sci, Chiba 2748510, Japan

[2] Toho Univ, Med Ctr, Sakura Hosp, Sakura 2858741, Japan

[3] Sumitomo Elect Ind Ltd, Sakae Ku, Yokohama, Kanagawa 2448588, Japan

[4] Toho Univ, Fac Sci, Proteome Anal Ctr, Chiba 2748510, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 420卷 / 04期

关键词：

Conjugation; Glycine-N-acyltransferase; Glutamine-N-acyltransferase; Hepatocellular carcinoma; Human; ACYL-COA-GLYCINE; HUMAN-LIVER; IDENTIFICATION; PURIFICATION; EXPRESSION; ESTERS; ACID;

D O I：

10.1016/j.bbrc.2012.03.099

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human glycine-N-acyltransferase (hGLYAT) gene and two related-genes (GLYATL1 and GLYATL2) were isolated. Human GLYAT, GLYATL1, and GLYATL2 cDNAs were isolated and shown to encode polypeptides of 295, 302, and 294 amino acids, respectively. GLYAT catalyzes glycine-N-acyltransfer reaction with benzoyl-CoA acting as a typical aralkyl transferase, while GLYATL1 catalyzed glutamine-N-acyltransfer reaction with phenylacetyl-CoA as an arylacetyl transferase. GLYAT was shown to be expressed specifically in the liver and kidney, and the cellular localization of GLYAT protein was restricted to the mitochondria. Interestingly, labeling using highly affinity purified anti-GLYAT antibody revealed that GLYAT expression was suppressed in all hepatocellular carcinomas, but not in other liver diseases. hGLYAT repression in cancerous cells in the liver was controlled at the transcriptional level. hGLYAT is a good candidate as a novel marker of hepatocellular carcinoma and may be a key molecule in the transition between differentiation and carcinogenesis of liver cells. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：901 / 906

页数：6

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