Molecular characterization and expression analysis of the large yellow croaker (Larimichthys crocea) chemokine receptors CXCR2, CXCR3, and CXCR4 after bacterial and poly I:C challenge

被引：24
作者：
Liu, Xiaoxu [1 ]
Kang, Lisen [1 ]
Liu, Wei [1 ]
Lou, Bao [2 ]
Wu, Changwen [1 ]
Jiang, Lihua [1 ]
机构：
[1] Zhejiang Ocean Univ, Coll Marine Sci, Natl Engn Res Ctr Marine Facil Aquaculture, 1 Haida South Rd, Zhoushan 316022, Zhejiang, Peoples R China
[2] Marine Fishery Inst Zhejiang Prov, Key Lab Mariculturre & Enhancement Zhejiang Prov, Zhoushan 316021, Zhejiang, Peoples R China
来源：
FISH & SHELLFISH IMMUNOLOGY | 2017年 / 70卷
基金：
对外科技合作项目（国际科技项目）;
关键词：
Larimichthys crocea; Large yellow croaker; Chemokine receptor; CXCR family; Bacterial challenge; Marine fish; GENE-EXPRESSION; CLONING; INTERLEUKIN-8; CELLS; INFECTION; RESPONSES; SEQUENCE; SYSTEM; BRAIN; FISH;
D O I：
10.1016/j.fsi.2017.08.029
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The large yellow croaker (Larimichthys crocea) has a well-developed innate immune system. We studied a component of this system, chemokine receptor CXCR family. In this study, we report the full-length open reading frames, as well as the identification and characterization of the chemokine receptor genes CXCR2 (LycCXCR2), CXCR3 (LycCXCR3), and CXCR4 (LycCXCR4) of large yellow croaker. We report that LycCXCR3 and LycCXCR4 are evolving neutrally according to PAML analySes. Quantitative real-time PCR analysis revealed that CXCR transcripts were expressed in all examined tissues. The expression of chemokine receptors LycCXCR2, LycCXCR3, and LycCXCR4 was elevated in the kidney, spleen, and particularly the liver of the large yellow croaker after challenge with Vibrio anguillarum and polyinosinic:polycytidylic acid (poly I:C). These results suggest that LycCXCR2, LycCXCR3, and LycCXCR4 may be important immune related genes, playing crucial roles in immune defence against bacterial infection. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：228 / 239
页数：12
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