Protective effects of resveratrol on random-pattern skin flap survival: an experimental study

被引:3
作者
Lin, Jinti [1 ,2 ,3 ,4 ]
Lin, Renjin [1 ,2 ,3 ,4 ]
Li, Shihen [1 ,2 ,3 ,4 ]
Wu, Hongqiang [1 ,2 ,3 ,4 ]
Ding, Jian [1 ,2 ,3 ,4 ]
Xiang, Guangheng [1 ,2 ,3 ,4 ]
Li, Shi [1 ,2 ,3 ,4 ]
Wang, Yiru [5 ]
Lin, Dingsheng [1 ,2 ,3 ,4 ]
Gao, Weiyang [1 ,2 ,3 ,4 ]
Kong, Jianzhong [1 ,2 ,3 ,4 ]
Xu, Huazi [1 ,2 ,3 ,4 ]
Zhou, Kailiang [1 ,2 ,3 ,4 ]
机构
[1] Wenzhou Med Univ, Affiliated Hosp 2, Dept Orthopaed, Wenzhou 325027, Peoples R China
[2] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Peoples R China
[3] Zhejiang Prov Key Lab Orthopaed, Wenzhou 325027, Peoples R China
[4] Wenzhou Med Univ, Clin Med Coll 2, Wenzhou 325027, Peoples R China
[5] Wenzhou Tradit Chinese Med Hosp, Dept Neurol, Wenzhou 325000, Peoples R China
来源
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH | 2019年 / 11卷 / 01期
关键词
Resveratrol; random-pattern skin flap; angiogenesis; apoptosis; oxidative stress; autophagy; ISCHEMIA-REPERFUSION; FUNCTIONAL RECOVERY; LIPID-PEROXIDATION; OXIDATIVE STRESS; AUTOPHAGY; RATS; INJURY; NEOVASCULARIZATION; STIMULATION; OXYGENASE-1;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Random-pattern skin flap transplantation is a common procedure in plastic surgery, but its distal area usually incurs ischemia and necrosis. Resveratrol (Rev), a natural polyphenol primarily found in peanuts, grapes, and red wine, which exerts multi-bioactivity. In this study, forty-eight rats with the modified "McFarlane flap" model were divided into Control and Rev groups, which were treated with vehicle Control and Rev, respectively. After 7 days of continuous treatment and observation, ischemic flap tissues were harvested to evaluate angiogenesis, apoptosis, oxidative stress, and autophagy. It was observed a greater survival area of flaps, accompanied with reduced water content and stronger blood supply, in the Rev group than in the Control group. In addition, Rev upregulated the expression of MMP9, VEGF, and Cadherin5, indicating that Rev promotes angiogenesis in ischemic flaps. Moreover, Rev decreased the levels of Bax, CYC, and Caspase3, suggesting that it inhibits apoptosis. Besides, Rev increased the expression of SOD1, eNOS, HO1, the activities of SOD and GSH, and reduced the levels of MDA, which uncovers that it depresses oxidative stress in ischemic flaps. Finally, it increased the expression of Beclin1, LC3II, VPS34, and CTSD, and decreased SQSTM1/p62 levels, which reveals that it activates autophagy in the flaps. These results suggest that Rev promotes random skin flap survival through proangiogenic, antiapoptotic, and antioxidative effects; moreover, autophagy is activated in the process, which might be another underlying mechanism for the flap survival.
引用
收藏
页码:379 / 392
页数:14
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