Circ_0004712 promotes endometriotic epithelial cell proliferation, migration and invasion by regulating miR-488-3p/ROCK1 axis in vitro

被引:3
作者
Xu, Ke [1 ]
Chen, Yang [1 ]
Li, Shufen [2 ]
Chen, Li [1 ]
Huang, Xiaoyang [1 ,3 ]
机构
[1] Nanjing Med Univ, Changzhou Maternal & Child Hlth Care Hosp, Dept Reprod Ctr, Changzhou Med Ctr, Changzhou, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Changzhou Maternal & Child Hlth Care Hosp, Changzhou Med Ctr, Dept Gynecol, Changzhou, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Changzhou Maternal & Child Hlth Care Hosp, Dept Reprod Ctr, Changzhou Med Ctr, 16 Dingxiang Rd,Beigang St, Changzhou 213000, Jiangsu, Peoples R China
关键词
Circ_0004712; MiR-488-3p; ROCK1; Endometriosis; Epithelial cells; CIRCULAR RNAS; MESENCHYMAL TRANSITION; STROMAL CELLS; PATHOGENESIS;
D O I
10.1016/j.repbio.2022.100667
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Recent evidence indicates that circular RNAs (circRNAs) play crucial regulatory roles in the pathogenesis and development of endometriosis. Circ_0004712 was found to be differentially expressed in endometriosis. However, the detailed function and mechanism of circ_0004712 in endometriosis are still unclear. Quantitative realtime polymerase chain reaction and Western blot were used for the detection of circ_0004712, miR-488-3p and ROCK1 (Rho Associated Coiled-Coil Containing Protein Kinase 1) levels. In vitro experiments in endometrial endothelial cells were performed by cell counting kit-8, EdU, transwell, wound healing assays, and flow cytometry, respectively. The molecular mechanism of circ_0004712 function was investigated using bioinformatics target predication, dual-luciferase reporter, and RNA immunoprecipitation (RIP) assays. The expression of circ_0004712 was higher in endometriotic endometrial tissues and epithelial cells. Knockdown of circ_0004712 suppressed cell proliferation, migration, invasion, EMT process and induced apoptosis in ectopic endometrial epithelial cells in vitro. Mechanistically, circ_0004712 acted as a ceRNA to sponge miR-488-3p, thus elevating the expression of ROCK1, which was confirmed to be a target of miR-488-3p. Rescue experiments suggested that miR-488-3p inhibition reversed the inhibitory effects of circ_0004712 silencing on cell growth and metastasis. Moreover, miR-488-3p restoration restrained the proliferation and metastasis in ectopic endometrial epithelial cells, which were attenuated by ROCK1 overexpression. Circ_0004712 knockdown suppressed the proliferation and metastasis of ectopic endometrial epithelial cells via miR-488-3p/ROCK1 axis in vitro, suggesting a new insight into the pathogenesis of endometriosis.
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页数:10
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