A reverse transcription-free real-time PCR assay for rapid miRNAs quantification based on effects of base stacking

被引：17

作者：

Lu, Zhuoxuan ^{[1
]}

Duan, Demin ^{[1
]}

Cao, Rong ^{[1
]}

Zhang, Liming ^{[1
]}

Zheng, Kexiao ^{[1
]}

Li, Jiong ^{[1
]}

机构：

[1] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 26期

基金：

中国国家自然科学基金;

关键词：

MICRORNA EXPRESSION; RNA; CANCER; OLIGONUCLEOTIDES; LIGATION; PLATFORM; PROBES; SERUM;

D O I：

10.1039/c1cc10442j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A rapid and reverse transcription-free real-time PCR microRNA assay was developed based on effects of base stacking. This microRNA assay has been shown to be highly specific to homogenous miRNAs, and the procedure can be completed within 30 min starting from total RNA.

引用

页码：7452 / 7454

页数：3

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