Effects of S. mutans gene-modification and antibacterial monomer dimethylaminohexadecyl methacrylate on biofilm growth and acid production

被引：23

作者：

Chen, Hong ^{[1
,2
]}

Tang, Yunhao ^{[3
]}

Weir, Michael D. ^{[2
]}

Gao, Jianghong ^{[2
,4
]}

Imazato, Satoshi ^{[5
]}

Oates, Thomas W. ^{[2
]}

Lei, Lei ^{[1
]}

Wang, Suping ^{[2
,6
,7
]}

Hu, Tao ^{[1
]}

Xu, Hockin H. K. ^{[2
,8
,9
]}

机构：

[1] Sichuan Univ, West China Hosp Stomatol, Dept Prevent Dent, State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China

[2] Univ Maryland, Dept Adv Oral Sci & Therapeut, Dent Sch, Baltimore, MD 21201 USA

[3] Chongqing Med Univ, Dept Gastrointestinal Surg, Affiliated Hosp 2, Chongqing 400010, Peoples R China

[4] Xi An Jiao Tong Univ, Coll Stomatol, Clin Res Ctr Shaanxi Prov Dent & Maxillofacial Di, Dept Prevent Dent, Xian 710004, Peoples R China

[5] Osaka Univ, Dept Biomat Sci, Grad Sch Dent, Osaka, Japan

[6] Zhengzhou Univ, Dept Operat Dent & Endodont, Affiliated Hosp 1, Zhengzhou 450052, Henan, Peoples R China

[7] Zhengzhou Univ, Periodont & Stomatol Ctr, Affiliated Hosp 1, Zhengzhou 450052, Henan, Peoples R China

[8] Univ Maryland, Ctr Stem Cell Biol & Regenerat Med, Sch Med, Baltimore, MD 21201 USA

[9] Univ Maryland, Marlene & Stewart Greenebaum Canc Ctr, Sch Med, Baltimore, MD 21201 USA

来源：

DENTAL MATERIALS | 2020年 / 36卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Quaternary ammonium; Dimethylaminohexadecyl; Methacrylate; Streptococcus mutans; rnc gene modification; Lactic acid; Dental caries; STREPTOCOCCUS-MUTANS; QUATERNARY AMMONIUM; ANTIBIOTIC-RESISTANCE; BACTERIAL-RESISTANCE; RESIN COMPOSITES; VIRULENCE; CHLORHEXIDINE; TOLERANCE; SYSTEM; CARIES;

D O I：

10.1016/j.dental.2019.12.001

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objectives. Antibacterial quaternary ammonium monomers (QAMs) are used in resins. The rnc gene in Streptococcus mutans (S. mutans) plays a key role in resisting antibiotics. The objectives of this study were to investigate for the first time: (1) the effects of rnc deletion on S. mutans biofilms and acid production; (2) the combined effects of rnc deletion with dimethylaminohexadecyl methacrylate (DMAHDM) on biofilm-inhibition efficacy. Methods. Parent S. mutans strain UA159 (ATCC 700610) and the rnc-deleted S. mutans were used. Bacterial growth, minimum inhibitory concentration (MIC), and minimal bactericidal concentration (MBC) were measured to analyze the bacterial susceptibility of the parent and rnc-deleted S. mutans against DMAHDM, with the gold-standard chlorhexidine (CHX) as control. Biofilm biomass, polysaccharide and lactic acid production were measured. Results. The drug-susceptibility of the rnc-deleted S. mutans to DMAHDM or CHX was 2-fold higher than parent S. mutans. The drug-susceptibility did not increase after 10 passages (p < 0.05). Deleting the rnc gene increased the biofilm susceptibility to DMAHDM or CHX by 2-fold. The rnc-deletion in S. mutans reduced biofilm biomass, polysaccharide and lactic acid production, even at no drugs. DMAHDM was nearly 40 % more potent than the gold-standard CHX. The combination of rnc deletion+DMAHDM treatment achieved the greatest reduction in biofilm biomass, polysaccharide synthesis, and lactic acid production. Significance. Gene modification by deleting the rnc in S. mutans reduced the biofilm growth and acid production, and the rnc deletion+DMAHDM method showed the greatest biofilm-inhibition efficacy, for the first time. The dual strategy of antibacterial monomer+bacterial gene modification shows great potential to control biofilms and inhibit caries. (C) 2019 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.

引用

页码：296 / 309

页数：14

共 67 条

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