NOTCH1 signaling contributes to cell growth, anti-apoptosis and metastasis in salivary adenoid cystic carcinoma

被引：51

作者：

Su, Bo-Hua ^{[1
]}

Qu, Jing ^{[1
]}

Song, Min ^{[1
]}

Huang, Xiao-Yu ^{[1
]}

Hu, Xiao-Meng ^{[1
]}

Xie, Jian ^{[1
]}

Zhao, Yong ^{[2
]}

Ding, Lin-Can ^{[1
]}

She, Lin ^{[1
]}

Chen, Jiang ^{[3
]}

Lin, Li-Song ^{[4
]}

Lin, Xu ^{[5
]}

Zheng, Da-Li ^{[5
,6
]}

Lu, You-Guang ^{[1
]}

机构：

[1] Fujian Med Univ, Affiliated Stomatol Hosp, Dept Prevent Dent, Fuzhou, Peoples R China

[2] Fujian Med Univ, Affiliated Stomatol Hosp, Dept Pathol, Fuzhou, Peoples R China

[3] Fujian Med Univ, Affiliated Stomatol Hosp, Ctr Dent Implant, Fuzhou, Peoples R China

[4] Fujian Med Univ, Affiliated Hosp 1, Dept Oral & Maxillofacial Surg, Fuzhou, Peoples R China

[5] Fujian Med Univ, Sch Basic Med Sci, Key Lab, Minist Educ Gastrointestinal Canc, Fuzhou, Peoples R China

[6] Univ S Florida, H Lee Moffitt Canc Ctr, Tampa, FL 33682 USA

来源：

ONCOTARGET | 2014年 / 5卷 / 16期

关键词：

NOTCH1; salivary adenoid cystic carcinoma; proliferation; apoptosis; metastasis; HUMAN HEPATOCELLULAR-CARCINOMA; MICROARRAY DATA; CANCER CELLS; CYCLE ARREST; SURVIVAL; EXPRESSION; TRANSCRIPTION; TRANSFORMATION; PROLIFERATION; MODULATION;

D O I：

10.18632/oncotarget.2321

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Numerous studies have reported both the tumor-suppressive and oncogenic roles of the Notch pathway, indicating that Notch activity regulates tumor biology in a complex, context-dependent manner. The aim of the present study was to identify the role of NOTCH1 in the cell growth and metastasis of SACC. Methods: We analyzed the expression of NOTCH1 in clinical SACC samples using immunohistochemical staining. We silenced the expression of NOTCH1 and overexpressed activated NOTCH1 to elucidate the effects of NOTCH1 on proliferation, migration and invasion. NOTCH1 target genes were validated by real-time PCR. Results: Our results showed that NOTCH1 was upregulated in SACC tissues when compared with normal tissues, and this upregulation was further enhanced in SACC tissues with metastasis and recurrence when compared with SACC tissues without metastasis. Overexpression of NOTCH1 in SACC cells promoted cell growth, migration and invasion, and knockdown of NOTCH1 inhibited cell proliferation in vitro and tumorigenicity in vivo by inducing cell apoptosis. Conclusions: The results of this study suggest that NOTCH1 plays a key role in the cell growth, anti-apoptosis, and metastasis of SACC. NOTCH1 inhibitors might therefore have potential therapeutic applications in treating SACC patients by inhibiting cancer cell growth and metastasis.

引用

页码：6885 / 6895

页数：11

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