The effect of putrescine on DNA methylation on cabbage plant under salt stress conditions

被引:4
作者
Orhan, Emine [1 ]
Uzundumlu, Furkan [1 ]
Yigider, Esma [1 ]
Aydin, Murat [1 ]
机构
[1] Ataturk Univ, Fac Agr, Dept Agr Biotechnol, Erzurum, Turkey
关键词
Cabbage; CRED-RA; DNA methylation; putrescine; salt stress; SEEDLING GROWTH; L; GERMINATION; TOLERANCE; LEVEL;
D O I
10.3906/tar-1911-38
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Although studies on tolerance to abiotic stress conditions in cabbage species and varieties have been carried out, these studies have generally been conducted in field and greenhouse pot experiments. In such studies, morphological measurements and evaluations have been made. However, there are deficiencies in physiological and molecular detection studies. Therefore, this study focused on detecting any DNA methylation change in the genome of cabbage seedlings when NaCl (salt stress) in combination with putrescine (Put) was applied. Different concentrations of NaCl and Put were applied to white head cabbage (Brassica oleraceae E. cv Yalova-F1). Germination rate, root length, shoot length, seedling index and variation coefficient of germination timewere examined. Eight RAPD primers were used in the CRED-RA analysis. Polymorphism ratios were calculated for each concentration and averages were taken. The results showed that MspI enzyme used in the study was cut from the methylation site and the HpaII enzyme was cut when the methylation did not occur. A total seventy-eight methlation sensitive bands were determined based on the treatments. Class IV was the highest methylation type. This was followed by Class I, Class III and Class II, respectively. Total methylation rate (%) was varied from 59.0% to 71.8%. It was determined that the full methylation rate (%) was higher than hemi-methylation rate in all treatments. As a result of this study, it was determined that Put applied in both NaCl stress and stress-free conditions caused demethylation.
引用
收藏
页码:301 / 311
页数:11
相关论文
共 55 条
  • [1] High Salinity Induces Different Oxidative Stress and Antioxidant Responses in Maize Seedlings Organs
    AbdElgawad, Hamada
    Zinta, Gaurav
    Hegab, Mumtaz M.
    Pandey, Renu
    Asard, Han
    Abuelsoud, Walid
    [J]. FRONTIERS IN PLANT SCIENCE, 2016, 7
  • [2] Salt stress alters DNA methylation levels in alfalfa (Medicago spp)
    Al-Lawati, A.
    Al-Bahry, S.
    Victor, R.
    Al-Lawati, A. H.
    Yaish, M. W.
    [J]. GENETICS AND MOLECULAR RESEARCH, 2016, 15 (01):
  • [3] Polyamines: molecules with regulatory functions in plant abiotic stress tolerance
    Alcazar, Ruben
    Altabella, Teresa
    Marco, Francisco
    Bortolotti, Cristina
    Reymond, Matthieu
    Koncz, Csaba
    Carrasco, Pedro
    Tiburcio, Antonio F.
    [J]. PLANTA, 2010, 231 (06) : 1237 - 1249
  • [4] Role of putrescine in salt tolerance of Atropa belladonna plant
    Ali, RM
    [J]. PLANT SCIENCE, 2000, 152 (02) : 173 - 179
  • [5] The influence of exogenous capsaicin application on the germination, seedling growth, and yield of pepper
    Arin, Levent
    Arabaci, Cagatay
    [J]. TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, 2019, 43 (05) : 500 - 507
  • [6] Arslan E, 2012, THESIS
  • [7] Aydin M., 2015, Ziraat Fakultesi Dergisi, Ataturk Universitesi, V46, P43
  • [8] Aydin M., 2016, Yuzuncu Yil Universitesi Journal of Agricultural Sciences, V26, P319
  • [9] [崔辉梅 CUI Hnimei], 2006, [安徽农业科学, Journal of anhui agricultural sciences], V34, P4680
  • [10] Deveci M, 2017, AKAD ZIRAAT DERGISI, V6, P81