Insulin-binding aptamer-conjugated graphene oxide for insulin detection

被引：103

作者：

Pu, Ying ^{[1
,2
,3
]}

Zhu, Zhi ^{[2
,3
]}

Han, Da ^{[2
,3
]}

Liu, Huixia ^{[1
]}

Liu, Jun ^{[1
,2
,3
]}

Liao, Jie ^{[1
]}

Zhang, Kejing ^{[1
,2
,3
]}

Tan, Weihong ^{[2
,3
]}

机构：

[1] Cent S Univ, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China

[2] Univ Florida, Dept Chem, Gainesville, FL 32611 USA

[3] Univ Florida, Dept Physiol & Funct Genon, Gainesville, FL 32611 USA

来源：

ANALYST | 2011年 / 136卷 / 20期

基金：

美国国家卫生研究院;

关键词：

CARBON NANOTUBES; SELECTION; DNA; NANOPARTICLES; ENRICHMENT; MOLECULES; LIGANDS; CANCER; PROBES; CELLS;

D O I：

10.1039/c1an15407a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes a simple and sensitive aptamer/graphene oxide (GO) based assay for insulin detection. GO can protect DNA from nuclease cleavage, but aptamers can be detached from the GO surface by specific target binding. This exposes the aptamers to enzymatic cleavage and releases the target for a new cycle. Cycling of targets leads to significant signal amplification and low LOD.

引用

页码：4138 / 4140

页数：3

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