Molecular Diagnostics

被引：14

作者：

Choe, Hyonmin ^{[1
]}

Deirmengian, Carl A. ^{[2
]}

Hickok, Noreen J. ^{[3
]}

Morrison, Tiffany N. ^{[2
]}

Tuan, Rocky S. ^{[4
,5
]}

机构：

[1] Case Western Reserve Univ, Dept Orthopaed, Cleveland, OH 44106 USA

[2] Thomas Jefferson Univ, Rothman Inst, Dept Orthopaed Surg, Philadelphia, PA 19107 USA

[3] Thomas Jefferson Univ, Dept Orthopaed Surg, Philadelphia, PA 19107 USA

[4] Univ Pittsburgh, Sch Med, Ctr Cellular & Mol Engn, Pittsburgh, PA USA

[5] Univ Pittsburgh, Sch Med, Dept Orthopaed Surg, Pittsburgh, PA 15261 USA

来源：

JOURNAL OF THE AMERICAN ACADEMY OF ORTHOPAEDIC SURGEONS | 2015年 / 23卷

关键词：

POLYMERASE-CHAIN-REACTION; METHICILLIN-RESISTANT STAPHYLOCOCCUS; PROSTHETIC JOINT INFECTION; C-REACTIVE PROTEIN; BACTERIAL-INFECTION; TIME; PCR; METABOLOMICS; IDENTIFICATION; DNA;

D O I：

10.5435/JAAOS-D-14-00409

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Orthopaedic infections are complex conditions that require immediate diagnosis and accurate identification of the causative organisms to facilitate appropriate management. Conventional methodologies for diagnosis of these infections sometimes lack accuracy or sufficient rapidity. Molecular diagnostics is an emerging area of bench-to-bedside research in orthopaedic infections. Examples of promising molecular diagnostics include measurement of a specific biomarker in the synovial fluid, polymerase chain reaction-based detection of bacterial genes, and metabolomic determination of responses to orthopaedic infection.

引用

页码：S26 / S31

页数：6

共 42 条

[1] The Role of Metabolomics in Osteoarthritis Research [J].

Adams, Samuel B., Jr. ;

Setton, Lori A. ;

Nettles, Dana L. .

JOURNAL OF THE AMERICAN ACADEMY OF ORTHOPAEDIC SURGEONS, 2013, 21 (01) :63-64

[2] Enhancing nonmass lesions in the breast:: Evaluation with proton (1H) MR spectroscopy [J].

Bartella, Lia ;

Thakur, Sunitha B. ;

Morris, Elizabeth A. ;

Dershaw, D. David ;

Huang, Wei ;

Chough, Eugenia ;

Cruz, Maria C. ;

Liberman, Laura .

RADIOLOGY, 2007, 245 (01) :80-87

[3] Inflammatory Blood Laboratory Levels as Markers of Prosthetic Joint Infection A Systematic Review and Meta-Analysis [J].

Berbari, Elie ;

Mabry, Tad ;

Tsaras, Geoffrey ;

Spangehl, Mark ;

Erwin, Pat J. ;

Murad, Mohammad Hassan ;

Steckelberg, James ;

Osmon, Douglas .

JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2010, 92A (11) :2102-2109

[4] Detection of Periprosthetic Infections With Use of Ribosomal RNA-Based Polymerase Chain Reaction [J].

Bergin, Patrick F. ;

Doppelt, Jason D. ;

Hamilton, William G. ;

Mirick, Gudrun E. ;

Jones, Angela E. ;

Sritulanondha, Supatra ;

Helm, Jeannine M. ;

Tuan, Rocky S. .

JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2010, 92A (03) :654-663

[5] Simulated joint infection assessment by rapid detection of live bacteria with real-time reverse transcription polymerase chain reaction [J].

Birmingham, Patrick ;

Helm, Jeannine M. ;

Manner, Paul A. ;

Tuan, Rocky S. .

JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2008, 90A (03) :602-608

[6]

Choe H, 2014, POL J MICROBIOL, V63, P393

[7] Rapid sensitive molecular diagnosis of pyogenic spinal infections using methicillin-resistant Staphylococcus-specific polymerase chain reaction and 16S ribosomal RNA gene-based universal polymerase chain reaction [J].

Choe, Hyonmin ;

Aota, Yoichi ;

Kobayashi, Naomi ;

Nakamura, Yushi ;

Wakayama, Yusuke ;

Inaba, Yutaka ;

Saito, Tomoyuki .

SPINE JOURNAL, 2014, 14 (02) :255-262

[8] Use of Real-Time Polymerase Chain Reaction for the Diagnosis of Infection and Differentiation Between Gram-Positive and Gram-Negative Septic Arthritis in Children [J].

Choe, Hyonmin ;

Inaba, Yutaka ;

Kobayashi, Naomi ;

Aoki, Chie ;

Machida, Jiro ;

Nakamura, Naoyuki ;

Okuzumi, Shigeharu ;

Saito, Tomoyuki .

JOURNAL OF PEDIATRIC ORTHOPAEDICS, 2013, 33 (03) :E28-E33

[9] The Host Response: Toll-Like Receptor Expression in Periprosthetic Tissues as a Biomarker for Deep Joint Infection [J].

Cipriano, Cara ;

Maiti, Aparna ;

Hale, Gregory ;

Jiranek, William .

JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2014, 96A (20) :1692-1698

[10]

Deirmengian C, 2014, CLIN ORTHOP RELAT RE

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