Analytical Capabilities of the ImageStream Cytometer

被引：37

作者：

Zuba-Surma, Ewa K. ^{[1
]}

Ratajczak, Mariusz Z. ^{[2
]}

机构：

[1] Jagiellonian Univ, Dept Med Biotechnol, Fac Biochem Biophys & Biotechnol, Krakow, Poland

[2] Univ Louisville, James Graham Brown Canc Ctr, Stem Cell Biol Inst, Louisville, KY 40292 USA

来源：

RECENT ADVANCES IN CYTOMETRY, PART A: INSTRUMENTATION, METHODS, FIFTH EDITION | 2011年 / 102卷

关键词：

LASER-SCANNING CYTOMETRY; PLASMACYTOID DENDRITIC CELLS; HEMATOPOIETIC STEM-CELLS; BLOOD MONONUCLEAR-CELLS; FACTOR-KAPPA-B; FLOW-CYTOMETRY; BONE-MARROW; CYTOCHEMICAL METHODS; PROTEIN INTERACTIONS; ELECTRON-MICROSCOPY;

D O I：

10.1016/B978-0-12-374912-3.00008-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Imaging cytometry has recently become an important achievement in development of flow cytometric technologies. The ImageStream cytometer combines the vast features of classical flow cytometry including an impartial analysis of great number of cells in short period of time which results in strong statistical data output, with essential features of fluorescence microscopy such us collecting of real multiparameter images of analyzed objects. In this chapter, we would like to introduce an overview of imaging cytometry platform and emphasize the potential advantages of using this system for several experimental purposes. Moreover, both well established as well as potential applications of imaging cytometry will be described. Eventually, we would like to illustrate the unique use of ImageStream cytometer for identification and characterization of subpopulations of stem/ progenitor cells present in different biological specimens.

引用

页码：207 / 230

页数：24

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