A simple aptamer-based fluorescent assay for the detection of Aflatoxin B1 in infant rice cereal

被引：125
|
作者：
Chen, Lu [1 ,2 ,3 ]
Wen, Fang [1 ,3 ]
Li, Ming [1 ,3 ]
Guo, Xiaodong [1 ,3 ]
Li, Songli [1 ,3 ]
Zheng, Nan [1 ,3 ,4 ]
Wang, Jiaqi [1 ,3 ,4 ]
机构：
[1] Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr, Lab Qual & Safety Risk Assessment Dairy Prod Beij, Beijing 100193, Peoples R China
[2] Xinjiang Agr Univ, Coll Anim Sci, Urumqi 830052, Peoples R China
[3] Milk & Dairy Prod Inspect Ctr Beijing, Minist Agr, Beijing 100193, Peoples R China
[4] Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China
来源：
FOOD CHEMISTRY | 2017年 / 215卷
基金：
中国国家自然科学基金;
关键词：
Aflatoxin B-1; Aptamer; Fluorescent; Quenching; Infant rice cereal; UP-CONVERSION NANOPARTICLES; RESONANCE ENERGY-TRANSFER; LC-MS/MS; OCHRATOXIN-A; APTASENSOR; IMMUNOASSAY; MYCOTOXINS; SAMPLES; DETOXIFICATION; IMMUNOSENSOR;
D O I：
10.1016/j.foodchem.2016.07.148
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A fluorescent assay for the rapid, sensitive and specific detection of Aflatoxin B-1 (AFB(1)) was developed in this study. Initially, a DNA/DNA duplex was formed between a fluorescein-labeled AFB(1) aptamer and its partially complementary DNA strand containing a quencher moiety, resulting in fluorescence quenching due to the close proximity of fluorophore and quencher. Upon the addition of AFB(1), an aptamer/AFB(1) complex was generated to release the quencher-modified DNA strand, thus recovered the fluorescence of fluorescein and enabled quantitative detection for AFB(1) by monitoring fluorescence enhancement. Under optimized conditions, this assay exhibited a linear response to AFB(1) in the range of 5-100 ng/mL with a detection limit down to 1.6 ng/mL. Trials of this assay in infant rice cereal with satisfactory recovery in the range of 93.0%-106.8%, demonstrate that the new assay could be a potential sensing platform for AFB(1) determination in food. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：377 / 382
页数：6
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