A simple aptamer-based fluorescent assay for the detection of Aflatoxin B1 in infant rice cereal

被引：125
|
作者：
Chen, Lu [1 ,2 ,3 ]
Wen, Fang [1 ,3 ]
Li, Ming [1 ,3 ]
Guo, Xiaodong [1 ,3 ]
Li, Songli [1 ,3 ]
Zheng, Nan [1 ,3 ,4 ]
Wang, Jiaqi [1 ,3 ,4 ]
机构：
[1] Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr, Lab Qual & Safety Risk Assessment Dairy Prod Beij, Beijing 100193, Peoples R China
[2] Xinjiang Agr Univ, Coll Anim Sci, Urumqi 830052, Peoples R China
[3] Milk & Dairy Prod Inspect Ctr Beijing, Minist Agr, Beijing 100193, Peoples R China
[4] Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China
来源：
FOOD CHEMISTRY | 2017年 / 215卷
基金：
中国国家自然科学基金;
关键词：
Aflatoxin B-1; Aptamer; Fluorescent; Quenching; Infant rice cereal; UP-CONVERSION NANOPARTICLES; RESONANCE ENERGY-TRANSFER; LC-MS/MS; OCHRATOXIN-A; APTASENSOR; IMMUNOASSAY; MYCOTOXINS; SAMPLES; DETOXIFICATION; IMMUNOSENSOR;
D O I：
10.1016/j.foodchem.2016.07.148
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A fluorescent assay for the rapid, sensitive and specific detection of Aflatoxin B-1 (AFB(1)) was developed in this study. Initially, a DNA/DNA duplex was formed between a fluorescein-labeled AFB(1) aptamer and its partially complementary DNA strand containing a quencher moiety, resulting in fluorescence quenching due to the close proximity of fluorophore and quencher. Upon the addition of AFB(1), an aptamer/AFB(1) complex was generated to release the quencher-modified DNA strand, thus recovered the fluorescence of fluorescein and enabled quantitative detection for AFB(1) by monitoring fluorescence enhancement. Under optimized conditions, this assay exhibited a linear response to AFB(1) in the range of 5-100 ng/mL with a detection limit down to 1.6 ng/mL. Trials of this assay in infant rice cereal with satisfactory recovery in the range of 93.0%-106.8%, demonstrate that the new assay could be a potential sensing platform for AFB(1) determination in food. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：377 / 382
页数：6
相关论文
共 50 条
  • [21] Aptamer based surface plasmon resonance sensor for aflatoxin B1
    Sun, Linlin
    Wu, Liqing
    Zhao, Qiang
    MICROCHIMICA ACTA, 2017, 184 (08) : 2605 - 2610
  • [22] Aptamer-Based Fluorescent Assay for Sensitive Detection of Cardiac Troponin Ⅰ
    Yu Li
    Yuanzhan Yang
    Xuefei Lü
    Yulin Deng
    JournalofBeijingInstituteofTechnology, 2020, 29 (01) : 45 - 51
  • [23] Recent Advances in the Aptamer-Based Electrochemical Biosensors for Detecting Aflatoxin B1 and Its Pertinent Metabolite Aflatoxin M1
    Beitollahi, Hadi
    Tajik, Somayeh
    Dourandish, Zahra
    Zhang, Kaiqiang
    Quyet Van Le
    Jang, Ho Won
    Kim, Soo Young
    Shokouhimehr, Mohammadreza
    SENSORS, 2020, 20 (11) : 1 - 17
  • [24] Aptamer based fluorescence recovery assay for aflatoxin B1 using a quencher system composed of quantum dots and graphene oxide
    Lu, Zhisong
    Chen, Xuejuan
    Wang, Ying
    Zheng, Xinting
    Li, Chang Ming
    MICROCHIMICA ACTA, 2015, 182 (3-4) : 571 - 578
  • [25] GO-amplified fluorescence polarization assay for high-sensitivity detection of aflatoxin B1 with low dosage aptamer probe
    Ye, Hua
    Lu, Qianqian
    Duan, Nuo
    Wang, Zhouping
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2019, 411 (05) : 1107 - 1115
  • [26] Novel electrochemiluminescent assay for the aptamer-based detection of testosterone
    Canovas, Rocio
    Daems, Elise
    Campos, Rui
    Schellinck, Sofie
    Madder, Annemieke
    Martins, Jose C.
    Sobott, Frank
    De Wael, Karolien
    TALANTA, 2022, 239
  • [27] Fluorescence assay for aflatoxin B1 based on aptamer-binding triggered DNAzyme activity
    Cheng, Qiuyi
    Zhao, Qiang
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2024, 416 (28) : 6367 - 6375
  • [28] Development of aptamer fluorescent switch assay for aflatoxin B1 by using fluorescein-labeled aptamer and black hole quencher 1-labeled complementary DNA
    Li, Yapiao
    Sun, Linlin
    Zhao, Qiang
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2018, 410 (24) : 6269 - 6277
  • [29] Visual detection of aflatoxin B1 based on specific aptamer recognition combining with triple amplification strategy
    Zhang, Hongyan
    Mao, Weiwei
    Hu, Yijin
    Wei, Xiaohong
    Huang, Lishan
    Fan, Shen
    Huang, Mingqing
    Song, Yu
    Yu, Yuyan
    Fu, FengFu
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2022, 271
  • [30] FRET-based aptamer biosensor for selective and sensitive detection of aflatoxin B1 in peanut and rice
    Sabet, Fereshte Sadat
    Hosseini, Morteza
    Khabbaz, Hossein
    Dadmehr, Mehdi
    Ganjali, Mohammad Reza
    FOOD CHEMISTRY, 2017, 220 : 527 - 532
12345