Cytotoxicity and its test methodology for a bioabsorbable nitrided iron stent

被引:38
作者
Lin, Wenjiao [1 ]
Zhang, Gui [1 ]
Cao, Ping [2 ]
Zhang, Deyuan [1 ]
Zheng, Yufeng [3 ]
Wu, Rangxiu [1 ]
Qin, Li [1 ]
Wang, Geqi [1 ]
Wen, Taoyuan [1 ]
机构
[1] Lifetech Sci Shenzhen Co Ltd, R&D Ctr, Shenzhen 518057, Peoples R China
[2] Shenzhen Testing Ctr Med Devices, Shenzhen 518057, Peoples R China
[3] Peking Univ, Dept Mat Sci & Engn, Coll Engn, Beijing 100871, Peoples R China
关键词
bioabsorbable; nitrided iron stent; cytotoxicity; size effect; test methodology; IN-VITRO CORROSION; PURE IRON; INTRAVASCULAR ULTRASOUND; BIOCOMPATIBILITY; IMPLANTATION; DEGRADATION; BIOMATERIAL; RESISTANCE; TITANIUM; BEHAVIOR;
D O I
10.1002/jbm.b.33246
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Comprehensive assessments of the cytotoxicity of nitrided iron, a promising bioabsorbable metallic material, were conducted using in vitro methods. Extracting and standing experiments were conducted to determine the factors influencing the precipitation of the extract during extraction and incubation. The MTT method, fluorescent staining, and direct contact method were used to explore the in vitro cytotoxicity of nitrided iron stent extracts, nitrided iron foils, and their bulk corrosion products. The extracting and standing experiments confirmed that the extraction medium and available oxygen are crucial for precipitation during the extraction and incubation processes. In the MTT test, the extract of nitrided iron stents with a high iron ion concentration (124.11 +/- 7.55 g/mL) was not cytotoxic to L929 fibroblasts. Thus, the in vitro cytotoxicity of nitrided iron stents was actually caused by the size effect of corrosion particles and not the material itself. Test methodology for in vitro cytotoxicity of biodegradable iron-based materials was analyzed, and the results demonstrate that multiple methods should be combined for comprehensive evaluation of the cytocompatibility of bioabsorbable iron-based materials to get an impartial conclusion. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 764-776, 2015.
引用
收藏
页码:764 / 776
页数:13
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