Germline development in rat revealed by visualization and deletion of Prdm14

被引：20

作者：

Kobayashi, Toshihiro ^{[1
,2
]}

Kobayashi, Hisato ^{[3
]}

Goto, Teppei ^{[1
]}

Takashima, Tomoya ^{[4
]}

Oikawa, Mami ^{[1
]}

Ikeda, Hiroki ^{[3
]}

Terada, Reiko ^{[1
]}

Yoshida, Fumika ^{[1
]}

Sanbo, Makoto ^{[1
]}

Nakauchi, Hiromitsu ^{[5
,6
]}

Kurimoto, Kazuki ^{[3
]}

Hirabayashi, Masumi ^{[1
,2
]}

机构：

[1] Ctr Genet Anal Behav, Natl Inst Physiol Sci, Sect Mammalian Transgenesis, Okazaki, Aichi 4448787, Japan

[2] Grad Univ Adv Studies, Dept Physiol Sci, Okazaki, Aichi 4448787, Japan

[3] Nara Med Univ, Dept Embryol, Kashihara, Nara 6340813, Japan

[4] Tokyo Univ Agr, Dept Biosci, Setagaya Ku, Tokyo 1568502, Japan

[5] Univ Tokyo, Inst Med Sci, Div Stem Cell Therapy, Minato Ku, Tokyo 1088639, Japan

[6] Stanford Univ, Inst Stem Cell Biol & Regenerat Med, Dept Genet, Sch Med, Stanford, CA 94305 USA

来源：

DEVELOPMENT | 2020年 / 147卷 / 04期

基金：

日本学术振兴会;

关键词：

Prdm14; Primordial germ cell; Rat; EMBRYONIC STEM-CELLS; NAIVE PLURIPOTENCY; IN-VITRO; MOUSE; SPECIFICATION; FATE; GENERATION; LINEAGE; RECONSTITUTION; ESTABLISHMENT;

D O I：

10.1242/dev.183798

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Primordial germ cells (PGCs), the founder cells of the germline, are specified in pre-gastrulating embryos in mammals, and subsequently migrate towards gonads to mature into functional gametes. Here, we investigated PGC development in rats, by genetically modifying Prdm14, a unique marker and an essential PGC transcriptional regulator. We trace PGC development in rats, for the first time, from specification until the sex determination stage in fetal gonads using Prdm14 H2BVenus knock-in rats. We uncover that the crucial role of Prdm14 in PGC specification is conserved between rat and mice, by analyzing Prdm14-deficient rat embryos. Notably, loss of Prdm14 completely abrogates the PGC program, as demonstrated by failure of the maintenance and/or activation of germ cell markers and pluripotency genes. Finally, we profile the transcriptome of the post-implantation epiblast and all PGC stages in rat to reveal enrichment of distinct gene sets at each transition point, thereby providing an accurate transcriptional timeline for rat PGC development. Thus, the novel genetically modified rats and data sets obtained in this study will advance our knowledge on conserved versus species-specific features for germline development in mammals.

引用

页数：10

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